Isolation of wild microalgae from natural water bodies for high hydrogen producing strains

	Isolation of wild microalgae from natural water bodies for high hydrogen producing strains
	He, Meilin 1,2; Li, Ling 1; Liu, Jianguo 1
刊名	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
	2012-03-01
卷号	37 期号:5 页码:4046-4056
关键词	Water sampling Microalgal isolation Sulfur deprivation Nitrogen deprivation Hydrogen production Hydrogenase
ISSN号	0360-3199
通讯作者	Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China.
英文摘要	Water samples from various marine, freshwater and terrestrial environments in China have been collected and screened in our laboratory for wild clones of microalgae since 2008. Hundreds of microalgal clones were purified through plate streaking, of which 52 strains have been screened for their ability to produce H-2 under anaerobic conditions. Twenty eight strains including 13 freshwater green algae, 12 marine green algae and 3 cyanobacteria were found to evolve H-2 under sulfur or nitrogen deprivation. Parietochloris incisa, Chlorella protothecoides, Chlorella capsulata, Nannochloropsis sp., Tetraselmis helgolandica, Pyramimonas sp., Chlorella autotrophica, Dunaliella apiculata, Tetraselmis striata and Tetraselmis tetrathele were first reported as new H-2 producing species. Among those H-2 producing strains, freshwater C. protothecoides showed the highest H-2 producing capacity (2.93 H-2 ml/l/h) and accumulated up to 123.6 ml H-2 per liter culture under sulfur deprivation. Marine C. autotrophica and T. striata were two notable green marine species that produced H-2 phototrophically, with 3.73 ml/l and 20.11 ml/l of H-2 yield respectively. Our results also demonstrated that both the likelihood of a strain producing H-2, and the average H-2 output, in freshwater strains were much higher than that in marine strains. In addition, the time for inducing H-2 production in freshwater strains was short compared to the marine ones. All three Arthrospira strains tested were able to generate H-2 under sulfur (S-) deprivation though the cultures would quickly lose their vitality in S-deprived medium. Interestingly, Arthrospira produced H-2 under nitrogen deprivation, and the H-2 yield was even higher than under S-depletion. However, neither tested chrysophyte nor diatom produced any H-2 under anaerobiosis caused by S-deprivation. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Physical ; Electrochemistry ; Energy & Fuels
收录类别	SCI
原文出处	10.1016/j.ijhydene.2011.11.089
语种	英语
WOS记录号	WOS:000301615200010
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12231]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 10049, Peoples R China
推荐引用方式 GB/T 7714	He, Meilin,Li, Ling,Liu, Jianguo. Isolation of wild microalgae from natural water bodies for high hydrogen producing strains[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2012,37(5):4046-4056.
APA	He, Meilin,Li, Ling,&Liu, Jianguo.(2012).Isolation of wild microalgae from natural water bodies for high hydrogen producing strains.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,37(5),4046-4056.
MLA	He, Meilin,et al."Isolation of wild microalgae from natural water bodies for high hydrogen producing strains".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 37.5(2012):4046-4056.