Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris

	Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris
	Li, Rongfeng 1,2; Yu, Huahua1 ; Xing, Ronge1 ; Liu, Song 1; Qing, Yukun 1,2; Li, Kecheng 1,2; Li, Bing 1,2; Meng, Xiangtao 1,2; Cui, Jinhui 1,2; Li, Pengcheng1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2012-06-15
卷号	899 页码:86-95
关键词	Jellyfish Stomolophus meleagris Nematocyst proteins Shotgun NanoLC-MS/MS Proteomic analysis
ISSN号	1570-0232
通讯作者	Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	The nematocyst proteins of jellyfish Stomolophus meleagris, a complicated mixture, contain many important bioactive molecules. In present study, to gain comprehensive insight into the protein component and search some novel bioactive molecules in the nematocyst proteins, shotgun proteomic analysis of the nematocyst proteins was carried out by nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) for the first time. Digested peptides of the nematocyst proteins were analyzed by nanoLC-MS/MS and all MS/MS spectra were then automatically searched by the SEQUEST program. A total of 181 proteins had been identified, with the molecular weight ranging from 5268.06 to 843,487.57 and the pi from 4.49 to 11.39. Bioinformatic analysis was also applied to better understand the identified proteins. In the gene ontology (GO) annotation, all the identified proteins were classified into 13.9 and 7 groups according to biological process, cellular component and molecular function, respectively. Pathways analysis of the identified proteins was conducted with 33 corresponding pathways found. On the basis of pathways analysis, we also constructed the gene network to analyze the relationship of those genes each other, which contained enzyme-enzyme relation, protein-protein interaction and gene expression interaction. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods ; Chemistry, Analytical
收录类别	SCI
原文出处	10.1016/j.jchromb.2012.05.006
语种	英语
WOS记录号	WOS:000306247300013
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12213]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,899:86-95.
APA	Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Li, Pengcheng.(2012).Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,899,86-95.
MLA	Li, Rongfeng,et al."Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 899(2012):86-95.