The first kazal-type serine proteinase inhibitor in the swimming crab Portunus trituberculatus involved in immune response to bacteria and fungi

	The first kazal-type serine proteinase inhibitor in the swimming crab Portunus trituberculatus involved in immune response to bacteria and fungi
	Wang, Shuangyan 1,2; Cui, Zhaoxia 1; Liu, Yuan 1; Li, Qianqian1,2 ; Song, Chengwen1,2
刊名	AQUACULTURE
	2012-08-01
卷号	356 页码:55-60
关键词	Portunus trituberculatus Kazal type Serine proteinase inhibitor Expression profile Immunity
ISSN号	0044-8486
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	A cDNA clone, namely PtKPI, coding for a one-kazal domain serine proteinase inhibitor (SPI) was identified from haemocyte cDNA library of the swimming crab Portunus trituberculatus. It is the first kazal-type SPI reported from crabs, also the first one-kazal domain SPI in crustaceans. The full-length cDNA sequence was 683 bp and contained an open reading frame of 444 bp, 36 bp of 5'-untranslated region (UTR), and 203 bp of 3'-UTR with a poly(A) tail. It encoded a polypeptide of 147 amino acids with an estimated molecular mass of 15.85 kDa and a theoretical pI of 8.99. A signal peptide was defined at N-terminus with a putative cleavage site located after position 16 (GNA-YN). The domain of PtKPI with C-X3-C-X7-C-X6-Y-X3-C-X7-C-X12-C was similar to the consensus pattern of the kazal motif. One alpha-helix surrounded by an adjacent three-stranded beta-sheet constructed the classical three-dimensional structure of kazal domain. PtKPI transcript was detected at a high level in the eyestalk and haemocytes that are involved in immune function. Upon challenge with three different microorganisms Vibrio alginolyticus, Micrococcus luteus and Pichia pastoris, the temporal expression of PtKPI in haemocytes showed different activation times against bacteria and fungi within an experimental period of 72 h. These results indicate that PtKPI is potentially involved in acute response against invading bacteria and fungi in P. trituberculatus. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Fisheries ; Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1016/j.aquaculture.2012.05.036
语种	英语
WOS记录号	WOS:000306171100008
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12394]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,et al. The first kazal-type serine proteinase inhibitor in the swimming crab Portunus trituberculatus involved in immune response to bacteria and fungi[J]. AQUACULTURE,2012,356:55-60.
APA	Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,Li, Qianqian,&Song, Chengwen.(2012).The first kazal-type serine proteinase inhibitor in the swimming crab Portunus trituberculatus involved in immune response to bacteria and fungi.AQUACULTURE,356,55-60.
MLA	Wang, Shuangyan,et al."The first kazal-type serine proteinase inhibitor in the swimming crab Portunus trituberculatus involved in immune response to bacteria and fungi".AQUACULTURE 356(2012):55-60.