Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs

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	Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs
	Wang Shuxian 1; Wei Jianteng 2; Li Tianbao 1
刊名	JOURNAL OF BASIC MICROBIOLOGY
	2012-08-01
卷号	52 期号:4 页码:467-476
关键词	rpoS Susceptibility Environmental stresses Outer membrane proteins Vibrio alginolyticus
ISSN号	0233-111X
通讯作者	Wang, SX (reprint author), Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, 47 Guizhou Rd, Qingdao, Shandong, Peoples R China.
英文摘要	Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long-term starvation and that the LD50 of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS-PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
学科主题	Microbiology
收录类别	SCI
原文出处	10.1002/jobm.201100249
语种	英语
WOS记录号	WOS:000307162200012
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12389]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang Shuxian,Wei Jianteng,Li Tianbao. Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs[J]. JOURNAL OF BASIC MICROBIOLOGY,2012,52(4):467-476.
APA	Wang Shuxian,Wei Jianteng,&Li Tianbao.(2012).Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs.JOURNAL OF BASIC MICROBIOLOGY,52(4),467-476.
MLA	Wang Shuxian,et al."Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs".JOURNAL OF BASIC MICROBIOLOGY 52.4(2012):467-476.