Molecular characterization of Cynoglossus semilaevis CD28

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	Molecular characterization of Cynoglossus semilaevis CD28
	Hu, Yong-hua; Sun, Bo-guang; Deng, Tian; Sun, Li
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-05-01
卷号	32 期号:5 页码:934-938
关键词	Cynoglossus semilaevis CD28 T cell activation
ISSN号	1050-4648
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	T lymphocyte activation requires a combination of signals, one of which is provided by interaction between CD28 and its ligands on antigen presenting cells. Although CD28-like sequences have been identified in a few teleosts, the function of fish CD28 is virtually unknown. In this study, we cloned and analyzed a CD28 gene, CsCD28, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsCD28 contains 229 residues and shares 20.2%-40.3% overall sequence identities with known fish CD28 sequences. CsCD28 possesses structural features conserved in mammalian and teleost CD28, which include the MYPPPY motif in the extracellular immunoglobulin-like domain and the YXN motif in the cytoplasmic domain. The CsCD28 gene is 2746 bp and composed of four exons and three introns, which in organization resemble those of mammalian and trout CD28. Quantitative real time RT-PCR analysis showed that CsCD28 expression occurred predominately in kidney, spleen, gut, and gill. CsCD28 is localized on the surface of head kidney lymphocytes, and antibody ligation of CsCD28 induced significant levels of cellular proliferation. Taken together, these results indicate that CsCD28 is similar to mammalian CD28 in genetic and protein structures and possibly plays a role in T cell activation. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.02.021
语种	英语
WOS记录号	WOS:000302667200038
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12362]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Sun, Bo-guang,Deng, Tian,et al. Molecular characterization of Cynoglossus semilaevis CD28[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):934-938.
APA	Hu, Yong-hua,Sun, Bo-guang,Deng, Tian,&Sun, Li.(2012).Molecular characterization of Cynoglossus semilaevis CD28.FISH & SHELLFISH IMMUNOLOGY,32(5),934-938.
MLA	Hu, Yong-hua,et al."Molecular characterization of Cynoglossus semilaevis CD28".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):934-938.