Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix

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	Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix
	Wang, Chao 1,2; Wang, Hongxia 1; Li, Yan3 ; Liu, Baozhong1
刊名	MOLECULAR BIOLOGY REPORTS
	2012-04-01
卷号	39 期号:4 页码:5017-5024
关键词	Meretrix meretrix SNP FBA gene Growth trait
ISSN号	0301-4851
通讯作者	Liu, BZ (reprint author), Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	This study investigated whether there were single nucleotide polymorphisms (SNPs) in fructose-1,6-bisphosphate aldolase (FBA) gene associated with growth traits of the clam Meretrix meretrix. A FBA gene was identified in M. meretrix and its deduced amino acid residues shared high identity with type I aldolase. The FBA (MmeFBA) mRNA expression profile was examined by real-time PCR in different tissues and the significantly high expression level in foot and adduct muscle suggests that MmeFBA is a muscle type aldolase which functions in glycolytic pathway. In the MmeFBA gene, we identified four intron SNPs and three exon SNPs including a nonsynonymous SNP (mmfbae-2). These SNPs were genotyped in 205 clams from two clam populations with significantly different growth performance. Results showed that allele frequencies of three SNPs (mmfbai-1, mmfbai-3 and mmfbae-2) and the genotype frequency of mmfbai-1 were all significantly different between the two populations. The haplotype analysis further supported the three SNPs distributed differently between the two populations. This study successively characterized three growth-related SNPs in a gene involved in energy metabolism of M. meretrix. These findings could contribute the development of phenotype-selective breeding program in M. meretrix.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
原文出处	10.1007/s11033-011-1298-9
语种	英语
WOS记录号	WOS:000301108500187
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12348]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Queensland, Sch Biol Sci, St Lucia, Qld 4072, Australia
推荐引用方式 GB/T 7714	Wang, Chao,Wang, Hongxia,Li, Yan,et al. Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):5017-5024.
APA	Wang, Chao,Wang, Hongxia,Li, Yan,&Liu, Baozhong.(2012).Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix.MOLECULAR BIOLOGY REPORTS,39(4),5017-5024.
MLA	Wang, Chao,et al."Identification of a fructose-1,6-bisphosphate aldolase gene and association of the single nucleotide polymorphisms with growth traits in the clam Meretrix meretrix".MOLECULAR BIOLOGY REPORTS 39.4(2012):5017-5024.