Genomic sequence-based discovery of novel angucyclinone antibiotics from marine Streptomyces sp W007

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	Genomic sequence-based discovery of novel angucyclinone antibiotics from marine Streptomyces sp W007
	Zhang, Hongyu 1,4; Wang, Hongbo 3; Wang, Yipeng 1; Cui, Hongli 1; Xie, Zeping 1; Pu, Yang 1; Pei, Shiqian 3; Li, Fuchao 2; Qin, Song 1
刊名	FEMS MICROBIOLOGY LETTERS
	2012-07-01
卷号	332 期号:2 页码:105-112
关键词	genome sequence polyketide antifungal activity cytotoxicity angucyclinone antibiotics
ISSN号	0378-1097
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
英文摘要	A large number of novel bioactive compounds were discovered from microbial secondary metabolites based on the traditional bioactivity screenings. Recent fermentation studies indicated that the crude extract of marine Streptomyces sp. W007 possessed great potential in agricultural fungal disease control against Phomopsis asparagi, Polystigma deformans, Cladosporium cucumerinum, Monilinia fructicola, and Colletotrichum lagenarium. To further evaluate the biosynthetic potential of secondary metabolites, we sequenced the genome of Streptomyces sp. W007 and analyzed the identifiable secondary metabolite gene clusters. Moreover, one gene cluster with type II PKS implied the possibility of Streptomyces sp. W007 to produce aromatic polyketide of angucyclinone antibiotics. Therefore, two novel compounds, 3-hydroxy-1-keto-3-methyl-8-methoxy-1,2,3,4-tetrahydro-benz[a]anthracene and kiamycin with potent cytotoxicities against human cancer cell lines, were isolated from the culture broth of Streptomyces sp. W007. In addition, other four known angucyclinone antibiotics were obtained. The gene cluster for these angucyclinone antibiotics could be assigned to 20 genes. This work provides powerful evidence for the interplay between genomic analysis and traditional natural product isolation research.
学科主题	Microbiology
收录类别	SCI
原文出处	10.1111/j.1574-6968.2012.02582.x
语种	英语
WOS记录号	WOS:000305334300003
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12336]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Yantai Univ, Sch Pharm, Dept Pharmacol, Yantai, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hongyu,Wang, Hongbo,Wang, Yipeng,et al. Genomic sequence-based discovery of novel angucyclinone antibiotics from marine Streptomyces sp W007[J]. FEMS MICROBIOLOGY LETTERS,2012,332(2):105-112.
APA	Zhang, Hongyu.,Wang, Hongbo.,Wang, Yipeng.,Cui, Hongli.,Xie, Zeping.,...&Qin, Song.(2012).Genomic sequence-based discovery of novel angucyclinone antibiotics from marine Streptomyces sp W007.FEMS MICROBIOLOGY LETTERS,332(2),105-112.
MLA	Zhang, Hongyu,et al."Genomic sequence-based discovery of novel angucyclinone antibiotics from marine Streptomyces sp W007".FEMS MICROBIOLOGY LETTERS 332.2(2012):105-112.