Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

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	Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus
	Hu, Yong-hua; Deng, Tian; Sun, Bo-guang; Sun, Li
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-06-01
卷号	32 期号:6 页码:1155-1161
关键词	Vibrio harveyi Attenuated vaccine Rifampicin resistance Immersion vaccine
ISSN号	1050-4648
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Vibrio harveyi is a Gram-negative bacterial pathogen that can infect a wide range of marine animals. In previous studies, we have reported a virulent V. harveyi strain, T4D. In the present study, an attenuated mutant of T4D, T4DM, was obtained by selection of rifampicin resistance. Compared to the wild type, T4DM was different in whole-cell protein profile and much slower in growth rate when cultured in stress conditions caused by iron depletion. Virulence analysis showed that compared to T4D, T4DM exhibited a dramatically increased median lethal dose, impaired tissue dissemination capacity, defective hemolytic activity, and significantly reduced resistance against the killing effect of host serum. To examine the potential of T4DM as a live attenuated vaccine, Japanese flounder (Paralichthys olivaceus) were vaccinated with T4DM via intraperitoneal injection or immersion. The results showed that at one and two months post-vaccination, fish administered with T4DM via both approaches, in particular that of immersion, were effectively protected against not only V. harveyi but also Vibrio alginolyticus, another important fish pathogen. Microbiological analysis showed that following immersion vaccination, T4DM was recovered from the internal organs of the vaccinated fish in a time-dependent manner within the first 6 days post-vaccination. Serum antibodies against V. harveyi and V. alginolyticus were detected in T4DM-vaccinated fish, and, compared to serum from control fish, serum from T4DM-vaccinated fish was significantly enhanced in bactericidal activity. These results indicate that T4DM is an attenuated strain with residual infectivity and that T4DM can induce effective cross-species protection against both V. harveyi and V. alginolyticus when used as a live immersion vaccine. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.03.032
语种	英语
WOS记录号	WOS:000304511800026
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12311]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yong-hua,Deng, Tian,Sun, Bo-guang,et al. Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(6):1155-1161.
APA	Hu, Yong-hua,Deng, Tian,Sun, Bo-guang,&Sun, Li.(2012).Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus.FISH & SHELLFISH IMMUNOLOGY,32(6),1155-1161.
MLA	Hu, Yong-hua,et al."Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus".FISH & SHELLFISH IMMUNOLOGY 32.6(2012):1155-1161.