Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis

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	Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
	Yang, Jialong 1,3; Wei, Xiumei 2; Liu, Xiangquan 2; Xu, Jie 2; Yang, Dinglong 2; Yang, Jianmin 2; Fang, Jinghui 2; Hu, Xiaoke 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-04-01
卷号	32 期号:4 页码:578-585
关键词	Solen grandis Sialic acid-binding lectin Innate immunity Sugar recognition Real-time PCR
ISSN号	1050-4648
通讯作者	Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.
英文摘要	Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by beta-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.01.012
语种	英语
WOS记录号	WOS:000301827100009
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12301]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jialong,Wei, Xiumei,Liu, Xiangquan,et al. Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(4):578-585.
APA	Yang, Jialong.,Wei, Xiumei.,Liu, Xiangquan.,Xu, Jie.,Yang, Dinglong.,...&Hu, Xiaoke.(2012).Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(4),578-585.
MLA	Yang, Jialong,et al."Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.4(2012):578-585.