A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis

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	A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis
	Zhou, Z.1,2; Wang, L.1 ; Kong, P.1,2; Qiu, L.1; Zhang, H.1 ; Gao, Y.1,2 ; Yang, J.1,2; Song, L.1
刊名	INTERNATIONAL JOURNAL OF IMMUNOGENETICS
	2012-02-01
卷号	39 期号:1 页码:46-54
关键词	GABA(A) RECEPTORS GABA(A)-RECEPTOR-ASSOCIATED PROTEIN SURFACE EXPRESSION CRYSTAL-STRUCTURE BINDING MODE GABARAP APOPTOSIS AUTOPHAGY GATE-16 LIGAND
ISSN号	1744-3121
通讯作者	Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	The ?-aminobutyrate type A receptor-associated protein (GABARAP) is a ubiquitin-like modifier, which is implicated in membrane trafficking and fusion events of ?-aminobutyrate type A receptor, autophagy and apoptosis. In the present study, the gene encoding GABARAP (designated EsGABARAP) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends approach and expression sequence tag (EST) analysis. The full-length cDNA of EsGABARAP was of 457 bp, containing a 5' untranslated region (UTR) of 77 bp, a 3' UTR of 32 bp with a poly(A) tail and an open reading frame (ORF) of 348 bp encoding a polypeptide of 116 amino acids with the predicted molecular weight of 13.81 kDa and theoretical isoelectric point of 8.73. The deduced amino acid sequence of EsGABARAP shared higher similarity (91.897.4%) with those of other GABARAPs, and it contained a conserved MAP1_LC3 domain. In the phylogenetic tree, EsGABARAP was firstly clustered with GABARAPs from other animals and then gathered together with the same family proteins of GABARAP. The mRNA expression level of EsGABARAP in six tissues and its temporal expression level in haemocytes of crabs challenged with Listonella anguillarum were determined by quantitative real-time RT-PCR. The mRNA transcripts of EsGABARAP could be detected ubiquitously in the examined tissues, including haemocytes, hepatopancreas, muscle, gill, heart and gonad, with the highest expression level in hepatopancreas. The expression level of EsGABARAP mRNA in haemocytes was up-regulated after L.similar to anguillarum challenge and reached 6.58-fold of that in blank group at 24 h (P < 0.05) and 7.52-fold at 48 h (P < 0.05). The increasing transcripts in haemocytes after L.similar to anguillarum challenge gave the preliminary evidence for the involvement of EsGABARAP as a part of immune response against bacteria challenge in crabs.
学科主题	Genetics & Heredity ; Immunology
收录类别	SCI
原文出处	10.1111/j.1744-313X.2011.01044.x
语种	英语
WOS记录号	WOS:000298791300008
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12267]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Z.,Wang, L.,Kong, P.,et al. A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis[J]. INTERNATIONAL JOURNAL OF IMMUNOGENETICS,2012,39(1):46-54.
APA	Zhou, Z..,Wang, L..,Kong, P..,Qiu, L..,Zhang, H..,...&Song, L..(2012).A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis.INTERNATIONAL JOURNAL OF IMMUNOGENETICS,39(1),46-54.
MLA	Zhou, Z.,et al."A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis".INTERNATIONAL JOURNAL OF IMMUNOGENETICS 39.1(2012):46-54.