Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa

doi:10.1016/S1875-5364(21)60030-4

	Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa
	Yao Shuai 1; Zhang Jian-Qing 1; Hou Jin-Jun 1; Hu Xiao-Su 1; Wang Ling 1; Da Juan 1; Rao Wei 2; Wang Dan-Dan 2; Huang Yong 1; Wu Wan-Ying 1
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2021-04-01
卷号	19 期号:4 页码:295-304
关键词	High performance thin-layer chromatography Mass spectrometry QDa Ganoderma Authentication
ISSN号	2095-6975
DOI	10.1016/S1875-5364(21)60030-4
通讯作者	Wu Wan-Ying(wanyingwu@simm.ac.cn) ; Guo De-An(daguo@simm.ac.cn)
英文摘要	In this study, a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa (HPTLC-QDa) method for robust authentication of Ganoderma lucidum, a popular and valuable herbal medicine, has been developed. This method is simple and practical, which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface. The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid (5 : 5 : 0.2, V/V) and all bands were transferred to QDa system directly in situ using 80% methanol with 0.1% formic acid as desorption solvent. The acquired HPTLC-QDa spectra showed that luminous yellow band b3, containing ganoderic acid B/G/H and ganodeneric acid B, the major active components of Ganoderma, could be found only in G. lucidum and G. lucidum (Antler-shaped), but not in G. sinense and G. applanatum. Moreover, bands b13 and b14 with m/z 475/477 and m/z 475/491/495, respectively, could be detected in G. lucidum (Antler-shaped), but not in G. lucidum, thus allowing simple and robust authentication of G. lucidum with confused species. This method is proved to be simple, practical and reproducible, which can be extended to analyze other herbal medicines.
资助项目	National Key R&D Program of China[2019YFC1711000] ; National Key R&D Program of China[2018YFC1707900] ; National Natural Science Foundation of China[8200140730] ; Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program (2018)
WOS关键词	CHINESE HERBAL MEDICINES ; GAS-CHROMATOGRAPHY ; QUALITY-CONTROL ; IDENTIFICATION ; LUCIDUM ; TRITERPENOIDS ; LINGZHI ; FINGERPRINTS ; MORPHOLOGY ; RADIX
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
出版者	CHINESE JOURNAL NATURAL MEDICINES
WOS记录号	WOS:000641160300005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296546]
专题	中国科学院上海药物研究所
通讯作者	Wu Wan-Ying; Guo De-An
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Waters Technol Shanghai Co Ltd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yao Shuai,Zhang Jian-Qing,Hou Jin-Jun,et al. Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2021,19(4):295-304.
APA	Yao Shuai.,Zhang Jian-Qing.,Hou Jin-Jun.,Hu Xiao-Su.,Wang Ling.,...&Guo De-An.(2021).Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa.CHINESE JOURNAL OF NATURAL MEDICINES,19(4),295-304.
MLA	Yao Shuai,et al."Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa".CHINESE JOURNAL OF NATURAL MEDICINES 19.4(2021):295-304.