Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers

doi:10.1021/jacs.0c05379

	Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers
	Chai, Xianzhi 4,5; Han, Hai-Hao 1,4,5; Sedgwick, Adam C.2; Li, Na 3; Zang, Yi 1; James, Tony D.6; Zhang, Junji 4,5; Hu, Xi-Le 4,5; Yu, Yang 3; Li, Yao 3
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2020-10-21
卷号	142 期号:42 页码:18005-18013
ISSN号	0002-7863
DOI	10.1021/jacs.0c05379
通讯作者	Zhang, Junji(zhangjunji@ecust.edu.cn) ; Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要	Here, we report a beta-galactosidase (beta-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind to human serum albumin (HSA) to form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led to an increase in fluorescence emission (520 nm) corresponding to the binding of the fluorescent naphthalimide unit with HSA. In addition, this enabled visualization of the spiropyran fluorescence emission in aqueous media. Our probe/protein hybrid approach afforded a unique imaging platform with enhanced cell permeability and solubility that was capable of visualizing the cellular uptake of NpG@HSA before its activation by beta-Gal. The beta-Gal-mediated cleavage of the galactose unit within the NpG@HSA hybrid resulted in the formation of NpM@HSA and an increase in red fluorescence emission (620 nm). The resultant merocyanine unit was then able to undergo photoisomerization (merocyanine <-> spiropyran) to facilitate STORM (i.e., stochastic optical reconstruction microscopy) imaging with minimal phototoxicity and excellent photostability/reversibility. Using STORM, NpG@HSA was able to determine the subcellular distribution of beta-Gal activity between cell lines with nanoscale precision. We believe that this system represents a versatile imaging platform for the design of photochromic fluorescent probes suitable for illuminating the precise location of disease-specific biomarkers in various cellular processes.
资助项目	NSFC[21788102] ; NSFC[91853201] ; NSFC[21878086] ; NSFC[21722801] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; National Key Sci-Tech Special Projects of Infection Diseases of China[2018ZX10732202] ; International Cooperation Program of the Shanghai Science and Technology Committee[17520750100] ; Shanghai Rising-Star Program[19QA1402500] ; Royal Society
WOS关键词	INTRAMOLECULAR SPIROCYCLIZATION ; POSTTRANSLATIONAL MODIFICATIONS ; IONIZING-RADIATION ; BETA-GALACTOSIDASE ; FLUOROPHORES ; SENESCENCE ; CELLS ; NANOPARTICLES
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000580559000024
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296465]
专题	中国科学院上海药物研究所
通讯作者	Zhang, Junji; Li, Jia; He, Xiao-Peng
作者单位	1.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China 2.Univ Texas Austin, Dept Chem, Austin, TX 78712 USA 3.Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 4.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China 5.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China 6.Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
推荐引用方式 GB/T 7714	Chai, Xianzhi,Han, Hai-Hao,Sedgwick, Adam C.,et al. Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(42):18005-18013.
APA	Chai, Xianzhi.,Han, Hai-Hao.,Sedgwick, Adam C..,Li, Na.,Zang, Yi.,...&Tian, He.(2020).Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(42),18005-18013.
MLA	Chai, Xianzhi,et al."Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.42(2020):18005-18013.