Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes

doi:10.3389/fendo.2020.592818

	Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes
	Duan, Ya-Nan 1,2; Ge, Xiang 3; Jiang, Hao-Wen 2; Zhang, Hong-Jie 4; Zhao, Yu 3; Li, Jin-Long 3,4; Zhang, Wei 5; Li, Jing-Ya 2
刊名	FRONTIERS IN ENDOCRINOLOGY
	2020-12-10
卷号	11 页码:13
关键词	diphyllin obesity brown adipocyte differentiation thermogenesis V-ATPase
ISSN号	1664-2392
DOI	10.3389/fendo.2020.592818
通讯作者	Li, Jin-Long(jinlongli@ntu.edu.cn) ; Zhang, Wei(wzhang@sat.ecnu.edu.cn) ; Li, Jing-Ya(jyli@simm.ac.cn)
英文摘要	Brown adipose tissue (BAT) and beige adipose tissue dissipate metabolic energy and mediate nonshivering thermogenesis, thereby boosting energy expenditure. Increasing the browning of BAT and beige adipose tissue is expected to be a promising strategy for combatting obesity. Through phenotype screening of C3H10-T1/2 mesenchymal stem cells, diphyllin was identified as a promising molecule in promoting brown adipocyte differentiation. In vitro studies revealed that diphyllin promoted C3H10-T1/2 cell and primary brown/beige preadipocyte differentiation and thermogenesis, which resulted increased energy consumption. We synthesized the compound and evaluated its effect on metabolism in vivo. Chronic experiments revealed that mice fed a high-fat diet (HFD) with 100 mg/kg diphyllin had ameliorated oral glucose tolerance and insulin sensitivity and decreased body weight and fat content ratio. Adaptive thermogenesis in HFD-fed mice under cold stimulation and whole-body energy expenditure were augmented after chronic diphyllin treatment. Diphyllin may be involved in regulating the development of brown and beige adipocytes by inhibiting V-ATPase and reducing intracellular autophagy. This study provides new clues for the discovery of anti-obesity molecules from natural products.
资助项目	National Natural Science Foundation of China[81673493] ; National Natural Science Foundation of China[81803384] ; National Science and Technology Major Project[2018ZX09711002-018] ; Natural Science Foundation of Jiangsu Province, China[BK20180947] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA12050409] ; Strategic Pilot Program of the Chinese Academy of Sciences[XDA12040204] ; Hong Kong Scholars program[XJ2019054]
WOS关键词	V-ATPASE INHIBITOR ; ADIPOSE-TISSUE ; PPAR-GAMMA ; AUTOPHAGY ; ACIDIFICATION ; THERMOGENESIS ; IMPAIRMENT ; ACTIVATION ; COLD
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000601263200001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296270]
专题	中国科学院上海药物研究所
通讯作者	Li, Jin-Long; Zhang, Wei; Li, Jing-Ya
作者单位	1.East China Normal Univ, Shanghai Engn Res Ctr Mol Therapeut & New Drug De, Sch Chem & Mol Engn, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Nantong Univ, Sch Pharm, Nantong, Peoples R China 4.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 5.East China Normal Univ, Kay Lab Brain Funct Genom, Minist Educ, Shanghai Key Lab Brain Funct Genom,Sch Life Sci, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Duan, Ya-Nan,Ge, Xiang,Jiang, Hao-Wen,et al. Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes[J]. FRONTIERS IN ENDOCRINOLOGY,2020,11:13.
APA	Duan, Ya-Nan.,Ge, Xiang.,Jiang, Hao-Wen.,Zhang, Hong-Jie.,Zhao, Yu.,...&Li, Jing-Ya.(2020).Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes.FRONTIERS IN ENDOCRINOLOGY,11,13.
MLA	Duan, Ya-Nan,et al."Diphyllin Improves High-Fat Diet-Induced Obesity in Mice Through Brown and Beige Adipocytes".FRONTIERS IN ENDOCRINOLOGY 11(2020):13.