Sleeve gastrectomy ameliorated high-fat diet (HFD)-induced non-alcoholic fatty liver disease and upregulated the nicotinamide adenine dinucleotide +/ Sirtuin-1 pathway in mice

doi:10.1016/j.asjsur.2020.05.030

	Sleeve gastrectomy ameliorated high-fat diet (HFD)-induced non-alcoholic fatty liver disease and upregulated the nicotinamide adenine dinucleotide +/ Sirtuin-1 pathway in mice
	Hua, Rong 2; Wang, Guan-Zhen 3; Shen, Qi-Wei 2; Yang, Ye-Ping 1; Wang, Meng 1; Wu, Meng 4; Shao, Yi-Kai 2; He, Min 1; Zang, Yi 3; Yao, Qi-Yuan 2
刊名	ASIAN JOURNAL OF SURGERY
	2021
卷号	44 期号:1 页码:213-220
关键词	NAD NAFLD NRK1 SIRT1 Sleeve gastrectomy
ISSN号	1015-9584
DOI	10.1016/j.asjsur.2020.05.030
通讯作者	Zang, Yi(yzang@simm.ac.cn) ; Yao, Qi-Yuan(hs_cbms@sina.cn) ; Zhang, Zhao-Yun(zhaoyunzhang@fudan.edu.cn)
英文摘要	Background: /Objective: Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease, and effective treatments are lacking. Bariatric surgery, including sleeve gastrectomy (SG), is a potential therapeutic strategy for NAFLD, but the molecular mechanisms underlying its effects are not fully understood. In this study, the effects of SG and the underlying mechanisms were evaluated in a mouse model of high-fat diet (HFD)-induced NAFLD. Methods: C57BL/6 mice were randomly divided into three groups: normal diet with sham operation (NC Sham group), HFD with sham operation (HFD-Sham group), and HFD with sleeve gastrectomy (HFD-SG group). Glucose metabolism and fat accumulation in the body and liver were analyzed before and after SG. Lipid metabolism and inflammation in the liver were evaluated. Nicotinamide adenine dinucleotide (NAD+) levels as well as nicotinamide riboside kinase (NRK1) and Sirtuin-1 (SIRT1) expression levels were evaluated. Results: SG attenuated the HFD-induced increases in glucose and insulin levels, fat accumulation, and lipid droplet accumulation. Fatty acid biosynthesis, the expression of the metabolism-related genes ACC1, FASN, SCD1, and DGAT1, and the levels of inflammatory factors were higher in HFD mice than in NC mice and decreased after SG. NAD + concentrations were 54.9 +/- 13.4 mmol/mg in NC-Sham mice, 37.6 +/- 8.1 mmol/mg in HFD-Sham mice, and 79.9 +/- 13.0 mmol/mg in HFD-SG mice (p < 0.05). NRK1 and SIRT1 expression increased dramatically after SG at both the RNA and protein levels. Conclusion: SG significantly alleviated NAFLD in HFD-induced obese mice with increasing the hepatic NAD + levels and upregulating the NRK1/NAD+/SIRT1 pathway. (C) 2020 Asian Surgical Association and Taiwan Robotic Surgery Association. Publishing services by Elsevier B.V.
资助项目	National Natural Science Foundation of China[31871414] ; National Natural Science Foundation of China[81673489] ; National Natural Science Foundation of China[81800751] ; National Natural Science Foundation of China[81970716] ; Science and Technology Commission of Shanghai Municipality[16430724100]
WOS关键词	BARIATRIC SURGERY ; NAD(+) ; SIRT1 ; STEATOHEPATITIS ; MONONUCLEOTIDE ; PATHOGENESIS ; METABOLISM ; DESIGN
WOS研究方向	Surgery
语种	英语
出版者	ELSEVIER SINGAPORE PTE LTD
WOS记录号	WOS:000616719600034
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/295591]
专题	中国科学院上海药物研究所
通讯作者	Zang, Yi; Yao, Qi-Yuan; Zhang, Zhao-Yun
作者单位	1.Fudan Univ, Huashan Hosp, Dept Endocrinol & Metab, Shanghai 200040, Peoples R China 2.Fudan Univ, Huashan Hosp, Dept Gen Surg, Shanghai 200040, Peoples R China 3.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Soochow Univ, Affiliated Hosp 2, Dept Endocrinol, Suzhou 215004, Peoples R China
推荐引用方式 GB/T 7714	Hua, Rong,Wang, Guan-Zhen,Shen, Qi-Wei,et al. Sleeve gastrectomy ameliorated high-fat diet (HFD)-induced non-alcoholic fatty liver disease and upregulated the nicotinamide adenine dinucleotide +/ Sirtuin-1 pathway in mice[J]. ASIAN JOURNAL OF SURGERY,2021,44(1):213-220.
APA	Hua, Rong.,Wang, Guan-Zhen.,Shen, Qi-Wei.,Yang, Ye-Ping.,Wang, Meng.,...&Zhang, Zhao-Yun.(2021).Sleeve gastrectomy ameliorated high-fat diet (HFD)-induced non-alcoholic fatty liver disease and upregulated the nicotinamide adenine dinucleotide +/ Sirtuin-1 pathway in mice.ASIAN JOURNAL OF SURGERY,44(1),213-220.
MLA	Hua, Rong,et al."Sleeve gastrectomy ameliorated high-fat diet (HFD)-induced non-alcoholic fatty liver disease and upregulated the nicotinamide adenine dinucleotide +/ Sirtuin-1 pathway in mice".ASIAN JOURNAL OF SURGERY 44.1(2021):213-220.