Clinical application of pyrrole-hemoglobin adducts as a biomarker of pyrrolizidine alkaloid exposure in humans

doi:10.1007/s00204-020-02947-4

	Clinical application of pyrrole-hemoglobin adducts as a biomarker of pyrrolizidine alkaloid exposure in humans
	Ma, Jiang 1,2; Zhang, Wei 3; He, Yisheng 1,2; Zhu, Lin 1,2; Zhang, Chunyuan 1,2; Liu, Jia 2,4; Ye, Yang 2,4; Zhuge, Yuzheng 3; Lin, Ge 1,2
刊名	ARCHIVES OF TOXICOLOGY
	2020-11-18
页码	7
关键词	Pyrrolizidine alkaloids Liver injury Diagnostic biomarkers Pyrrole-hemoglobin adducts Pyrrole-plasma protein adducts
ISSN号	0340-5761
DOI	10.1007/s00204-020-02947-4
通讯作者	Zhuge, Yuzheng(yuzheng9111963@aliyun.com) ; Lin, Ge(linge@cuhk.edu.hk)
英文摘要	Pyrrolizidine alkaloids (PAs) are naturally occurring hepatotoxins widely present in hundreds of plant species and also known to contaminate many foodstuffs, such as grain, honey, and tea. The formation of pyrrole-protein adducts via metabolic activation of PAs has been suggested as a primary trigger initiating hepatotoxicity. The present study for the first time tested the suitability of pyrrole-hemoglobin adducts as a novel and specific biomarker of PA exposure in humans. The level and elimination kinetics of pyrrole-hemoglobin adducts were systematically investigated in the blood samples of 43 PA-induced liver injury (PA-ILI) patients. The results revealed significantly higher concentrations (84.50 +/- 78.38 nM) and longer persistence (similar to 4 months) of pyrrole-hemoglobin adducts than that (concentration: 9.53 +/- 10.72 nM; persistence: similar to 2 months) of pyrrole-plasma protein adducts, our previously developed PA exposure biomarker. Our findings confirmed that pyrrole-hemoglobin adducts with higher level and longer persistence should serve as a more applicable PA exposure biomarker for future clinical diagnosis of PA-ILI in drug/herb-induced liver injury patients.
资助项目	Research Grants Council of Hong Kong[14111816] ; Research Grants Council of Hong Kong[14106318] ; Chinese University of Hong Kong[4054503]
WOS关键词	PROTEIN ADDUCTS ; METABOLIC-ACTIVATION ; ALBUMIN ADDUCTS ; LIVER TOXICITY ; STABILITY ; OXIDE ; FOOD
WOS研究方向	Toxicology
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000590483200001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292665]
专题	中国科学院上海药物研究所
通讯作者	Zhuge, Yuzheng; Lin, Ge
作者单位	1.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong, Peoples R China 2.Chinese Acad Sci, Joint Res Lab Promoting Globalizat Tradit Chinese, Shanghai, Peoples R China 3.Nanjing Univ, Sch Med, Drum Tower Hosp, Dept Gastroenterol, Nanjing, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Ma, Jiang,Zhang, Wei,He, Yisheng,et al. Clinical application of pyrrole-hemoglobin adducts as a biomarker of pyrrolizidine alkaloid exposure in humans[J]. ARCHIVES OF TOXICOLOGY,2020:7.
APA	Ma, Jiang.,Zhang, Wei.,He, Yisheng.,Zhu, Lin.,Zhang, Chunyuan.,...&Lin, Ge.(2020).Clinical application of pyrrole-hemoglobin adducts as a biomarker of pyrrolizidine alkaloid exposure in humans.ARCHIVES OF TOXICOLOGY,7.
MLA	Ma, Jiang,et al."Clinical application of pyrrole-hemoglobin adducts as a biomarker of pyrrolizidine alkaloid exposure in humans".ARCHIVES OF TOXICOLOGY (2020):7.