Machine-Driven Chemoenzymatic Synthesis of Glycopeptide

doi:10.1002/anie.202001124

	Machine-Driven Chemoenzymatic Synthesis of Glycopeptide
	Zhang, Jiabin 1; Liu, Ding 1; Saikam, Varma 1; Gadi, Madhusudhan R.1; Gibbons, Christopher 1; Fu, Xuan 1; Song, Heliang 1; Yu, Jin 4; Kondengaden, Shukkoor M.2; Wang, Peng G.1,2
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2020-08-31
页码	6
关键词	automation chemoenzymatic synthesis machine-driven peptide synthesizer silica resin
ISSN号	1433-7851
DOI	10.1002/anie.202001124
通讯作者	Wang, Peng G.(pwang11@gsu.edu) ; Wen, Liuqing(lwen@simm.ac.cn)
英文摘要	Historically, researchers have put considerable effort into developing automation systems to prepare natural biopolymers such as peptides and oligonucleotides. The availability of such mature systems has significantly advanced the development of natural science. Over the past twenty years, breakthroughs in automated synthesis of oligosaccharides have also been achieved. A machine-driven platform for glycopeptide synthesis by a reconstructed peptide synthesizer is described. The designed platform is based on the use of an amine-functionalized silica resin to facilitate the chemical synthesis of peptides in organic solvent as well as the enzymatic synthesis of glycan epitopes in the aqueous phase in a single reaction vessel. Both syntheses were performed by a peptide synthesizer in a semiautomated manner.
资助项目	National Institutes of Health[U01GM116263] ; Shanghai Institute of Materia Medica[2019000021]
WOS关键词	SOLID-PHASE PEPTIDE ; ENZYMATIC-SYNTHESIS ; ANTITUMOR VACCINES ; GLYCOSYLTRANSFERASE ; EXPRESSION ; INDUCTION
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000564021000001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292385]
专题	中国科学院上海药物研究所
通讯作者	Wang, Peng G.; Wen, Liuqing
作者单位	1.Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA 2.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Imperial Coll London, Du Cane Rd, London W12 0NN, England
推荐引用方式 GB/T 7714	Zhang, Jiabin,Liu, Ding,Saikam, Varma,et al. Machine-Driven Chemoenzymatic Synthesis of Glycopeptide[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020:6.
APA	Zhang, Jiabin.,Liu, Ding.,Saikam, Varma.,Gadi, Madhusudhan R..,Gibbons, Christopher.,...&Wen, Liuqing.(2020).Machine-Driven Chemoenzymatic Synthesis of Glycopeptide.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,6.
MLA	Zhang, Jiabin,et al."Machine-Driven Chemoenzymatic Synthesis of Glycopeptide".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020):6.