An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples

doi:10.1021/acs.analchem.0c00242

	An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples
	Liu, Huiying 1,2; Xu, Feng 1,2; Gao, Yuqing 3,4; Pang, Yuanyuan 1,2; Xie, Cen 3; Jiang, Changtao 1,2
刊名	ANALYTICAL CHEMISTRY
	2020-07-07
卷号	92 期号:13 页码:8810-8818
ISSN号	0003-2700
DOI	10.1021/acs.analchem.0c00242
通讯作者	Xie, Cen(xiecen@simm.ac.cn) ; Jiang, Changtao(jiangchangtao@bjmu.edu.cn)
英文摘要	The cellular redox balance plays a significant role in cell fate decisions and in the regulation of responses to various kinds of stress. In this study, we defined a novel concept of the oxidativeredox metabolome, and established a method for the simultaneous quantification of 23 metabolites involved in the oxidative-redox metabolome, covering NAD(+) pathway, FAD pathway, GSSG pathway, and ATP pathway by using the AB SCIEX 5500 QTRAP LC/MS/MS system. Corresponding oxidative-redox metabolomics analysis was performed in plasma of humans, hamsters and mice, and hamsters were demonstrated to display a stronger resemblance than mice to humans. The known reductant dithiothreitol (DTT) and oxidant hydrogen peroxide (H2O2) were selected to treat A549 and HeLa cells to validate the current method, showing that DTT moderately increased while H2O2 greatly decreased most analytes. Antibiotic treatment may disturb the oxidative-redox balance in vivo. By comparing the oxidative-redox metabolome in antibiotic-fed hamsters with that of control hamsters, we demonstrated a substantial metabolic disparity between the two, further verifying the applicability and reliability of our method.
资助项目	National Key Research and Development Program of China[2018YFA0800700] ; National Natural Science Foundation of China[91857115] ; National Natural Science Foundation of China[81700010] ; National Natural Science Foundation of China[81921001] ; National Natural Science Foundation of China[91957116] ; National Natural Science Foundation of China[31925021] ; Fundamental Research Funds for the Central Universities: Special Projects for Strengthening Basic Research of Peking University[BMU2019JC003] ; Clinical Medicine Plus X - Young Scholars Project Peking University[PKU2019LCXQ013] ; Fundamental Research Funds for the Central Universities, Shanghai Rising-Star Program[20QA1411200] ; Research Fund of State Key Laboratory of Drug Research[SIMM2004KF-04] ; Research Fund of State Key Laboratory of Drug Research[SIMM2003ZZ-02] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12050408] ; National Science and Technology Major Project of China[2018ZX09711002]
WOS关键词	GLUTATHIONE ; CELLS ; CYSTEINE ; STATE ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000548541200027
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292074]
专题	中国科学院上海药物研究所
通讯作者	Xie, Cen; Jiang, Changtao
作者单位	1.Peking Univ, Hosp 3, Ctr Basic Med Res, Inst Med Innovat & Res, Beijing 100191, Peoples R China 2.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100191, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huiying,Xu, Feng,Gao, Yuqing,et al. An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples[J]. ANALYTICAL CHEMISTRY,2020,92(13):8810-8818.
APA	Liu, Huiying,Xu, Feng,Gao, Yuqing,Pang, Yuanyuan,Xie, Cen,&Jiang, Changtao.(2020).An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples.ANALYTICAL CHEMISTRY,92(13),8810-8818.
MLA	Liu, Huiying,et al."An Integrated LC-MS/MS Strategy for Quantifying the Oxidative-Redox Metabolome in Multiple Biological Samples".ANALYTICAL CHEMISTRY 92.13(2020):8810-8818.