CryoEM structure of the tegumented capsid of Epstein-Barr virus

doi:10.1038/s41422-020-0363-0

	CryoEM structure of the tegumented capsid of Epstein-Barr virus
	Li, Zhihai 2; Zhang, Xiao 1; Dong, Lili 2; Pang, Jingjing 2,3; Xu, Miao 1; Zhong, Qian 1; Zeng, Mu-Sheng 1; Yu, Xuekui 2,3
刊名	CELL RESEARCH
	2020-07-03
页码	12
ISSN号	1001-0602
DOI	10.1038/s41422-020-0363-0
通讯作者	Zeng, Mu-Sheng(zengmsh@sysucc.org.cn) ; Yu, Xuekui(xkyu@simm.ac.cn)
英文摘要	Epstein-Barr virus (EBV) is the primary cause of infectious mononucleosis and has been shown to be closely associated with various malignancies. Here, we present a complete atomic model of EBV, including the icosahedral capsid, the dodecameric portal and the capsid-associated tegument complex (CATC). Our in situ portal from the tegumented capsid adopts a closed conformation with its channel valve holding the terminal viral DNA and with its crown region firmly engaged by three layers of ring-like dsDNA, which, together with the penton flexibility, effectively alleviates the capsid inner pressure placed on the portal cap. In contrast, the CATCs, through binding to the flexible penton vertices in a stoichiometric manner, accurately increase the inner capsid pressure to facilitate the pressure-driven genome delivery. Together, our results provide important insights into the mechanism by which the EBV capsid, portal, packaged genome and the CATCs coordinately achieve a pressure balance to simultaneously benefit both viral genome retention and ejection.
资助项目	National Key RD Program[2017YFA0505600] ; National Key RD Program[2016YFA0502101] ; Hundred Talents Program of Chinese Academy of Sciences ; Chinese Academy of Sciences[XDA12010317] ; Natural Science Foundation of Shanghai[18ZR1447700] ; National Natural Science Foundation of China[81830090] ; National Natural Science Foundation of China[31900869] ; National Natural Science Foundation of China[81702001] ; Shanghai Sailing Program[19YF1456800] ; China Postdoctoral Science Foundation[2019M651622]
WOS关键词	HERPES-SIMPLEX-VIRUS ; 3-DIMENSIONAL STRUCTURE ; VIRAL-DNA ; PROTEIN
WOS研究方向	Cell Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000545187300001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291938]
专题	中国科学院上海药物研究所
通讯作者	Zeng, Mu-Sheng; Yu, Xuekui
作者单位	1.Sun Yat Sen Univ, Canc Ctr, Dept Expt Res,Guangdong Key Lab Nasopharyngeal Ca, State Key Lab Oncol South China,Collaborat Innova, Guangzhou 510060, Guangdong, Peoples R China 2.Chinese Acad Sci, Cryoelectron Microscopy Res Ctr, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhihai,Zhang, Xiao,Dong, Lili,et al. CryoEM structure of the tegumented capsid of Epstein-Barr virus[J]. CELL RESEARCH,2020:12.
APA	Li, Zhihai.,Zhang, Xiao.,Dong, Lili.,Pang, Jingjing.,Xu, Miao.,...&Yu, Xuekui.(2020).CryoEM structure of the tegumented capsid of Epstein-Barr virus.CELL RESEARCH,12.
MLA	Li, Zhihai,et al."CryoEM structure of the tegumented capsid of Epstein-Barr virus".CELL RESEARCH (2020):12.