Exploring the protective effects of Danqi Tongmai tablet on acute myocardial ischemia rats by comprehensive metabolomics profiling

doi:10.1016/j.phymed.2019.152918

	Exploring the protective effects of Danqi Tongmai tablet on acute myocardial ischemia rats by comprehensive metabolomics profiling
	Li Zhenwei 2,3; Hou Jinjun 2; Deng Yanping 2; Zhi Haijuan 2; Wu Wenyong 2; Yan Bingpeng 1; Chen Tingting 4; Tu Jia 4; Zhu Zhengjiang 4; Wu Wanying 2
刊名	PHYTOMEDICINE
	2020-08-01
卷号	74 页码:9
关键词	Danqi Tongmai tablet Acute myocardial ischemia Metabolomics Lipidomics Network pharmacology
ISSN号	0944-7113
DOI	10.1016/j.phymed.2019.152918
通讯作者	Wu Wanying(wanyingwu@simm.ac.cn) ; Guo De-an(daguo@simm.ac.cn)
英文摘要	Background: Danqi Tongmai tablet (DQTM), a combination of salvianolic acids (SA) and panax notoginsenosides (PNS), is now in phase II clinical trial developed for the treatment of cardiovascular diseases. However, the mechanisms of its protective effects through regulating endogenous metabolites remain unclear. Purpose: The purpose of this study was to explore the protective effects of DQTM on acute myocardial ischemia rats by comprehensive metabolomics profiling. Study design: The rats were divided into three groups: sham-operating, acute myocardial ischemia (AMI) and DQTM groups. The plasma and heart were collected and profiled by LC-MS based metabolomics and lipidomics. Based on the identified differential metabolites, the pathway analysis results were obtained and further validated using the network pharmacology approach. Methods: The AMI model was induced by ligating the left anterior descending coronary artery. The metabolomics and lipidomics profiling were based on two established LC-QTOF/MS analysis methods. The raw data were processed using XCMS Online, then the differential metabolites with nonparametric t-test p value less than 0.05 were selected and identified using HMDB and METLIN. The pathway analysis was conducted using MetaboAnalyst and validated with the predicted network results obtained by BATMAN-TCM. Results: The metabolomics and lipidomics profiles of plasma and heart in response to AMI and DQTM were significantly different. The AMI operation had a serious influence on metabolites in heart ischemia region, while DQTM had a greater impact on lipids in heart non-ischemia region. A total of 151 differential metabolites were identified, including mainly amino acids and fatty acids. Multiple metabolic pathways were disturbed after AMI and could be restored by DQTM, of which arachidonic acid metabolism was further validated with the predicted results of network pharmacology. Conclusion: The protective effects of DQTM on acute myocardial ischemia rats could be achieved through the regulation of multiple metabolic pathways.
资助项目	National Natural Science Foundation of China[81530095] ; Shanghai Committee of Science and Technology[16DZ0500800]
WOS关键词	MASS-SPECTROMETRY ; SALVIANOLIC ACIDS ; NOTOGINSENGNOSIDES ; PLATFORM ; INJURY
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000538117000004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291633]
专题	中国科学院上海药物研究所
通讯作者	Wu Wanying; Guo De-an
作者单位	1.Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Pokfulam, Hong Kong, Peoples R China 2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Beijing 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Li Zhenwei,Hou Jinjun,Deng Yanping,et al. Exploring the protective effects of Danqi Tongmai tablet on acute myocardial ischemia rats by comprehensive metabolomics profiling[J]. PHYTOMEDICINE,2020,74:9.
APA	Li Zhenwei.,Hou Jinjun.,Deng Yanping.,Zhi Haijuan.,Wu Wenyong.,...&Guo De-an.(2020).Exploring the protective effects of Danqi Tongmai tablet on acute myocardial ischemia rats by comprehensive metabolomics profiling.PHYTOMEDICINE,74,9.
MLA	Li Zhenwei,et al."Exploring the protective effects of Danqi Tongmai tablet on acute myocardial ischemia rats by comprehensive metabolomics profiling".PHYTOMEDICINE 74(2020):9.