The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis

doi:10.1016/j.carres.2020.108093

	The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis
	Wang, Ying 1,2; Xiao, Fei 1,2; Jin, Can 1,2; Wang, Wucheng 1,2; Chen, Xia 1,2; Liu, Qin 1,2; Ding, Kan 1,2
刊名	CARBOHYDRATE RESEARCH
	2020-10-01
卷号	496 页码:10
关键词	Sulfated polysaccharides Bone morphogenic protein 2 Structure and activity relationship Angiogenesis
ISSN号	0008-6215
DOI	10.1016/j.carres.2020.108093
通讯作者	Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Sulfated polysaccharides play important roles in angiogenesis. However, the impact of structural alteration of sulfated polysaccharide on the bioactivity is still vague. In this study, binding between different sulfated polysaccharides and bone morphogenic protein 2 (BMP2) was measured to understand the sense of this motif transformation. The results showed that binding between sulfated alpha-1,4-glucan and BMP2 was the most intensive. The branch of alpha-1,4-glucan was important for the binding. The affinity of sulfated polysaccharides to BMP2 increased as the molecular weight (MW) and degree of substitution (DS) increased. DS that exceeded 1.05 impaired binding and played more important role in polysaccharide BMP2 interaction than MW. The reservation of partial 6-OH would benefit its binding ability to BMP2. Further, we showed that sulfated polysaccharides with strong binding to BMP2 blocked phosphorylation of Smad 1/5/8 and expression of Id1 to a greater extent than those not strongly bind to BMP2. The binding strength of polysaccharides to BMP2 increased, so did the potency of the anti-angiogenesis effects.
资助项目	Key New Drug Creation and Manufacturing Program, Ministry of Science and Technology[2019ZX09735001] ; National Key R&D Program of China, Ministry of Science and Technology[2019YFC1711000] ; key program of National Natural Science Foundation of China[31230022]
WOS关键词	DISRUPTING ANGIOGENESIS ; TARGETING VEGFR2/VEGF ; GROWTH ; DERIVATIVES ; EXPRESSION ; PROSTATE ; HEPARIN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000571835100004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291262]
专题	中国科学院上海药物研究所
通讯作者	Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ying,Xiao, Fei,Jin, Can,et al. The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis[J]. CARBOHYDRATE RESEARCH,2020,496:10.
APA	Wang, Ying.,Xiao, Fei.,Jin, Can.,Wang, Wucheng.,Chen, Xia.,...&Ding, Kan.(2020).The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis.CARBOHYDRATE RESEARCH,496,10.
MLA	Wang, Ying,et al."The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis".CARBOHYDRATE RESEARCH 496(2020):10.