Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue

doi:10.1016/j.redox.2020.101633

	Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue
	Aquilano, Katia 6; Sciarretta, Francesca 4; Turchi, Riccardo 6; Li, Bo-Han 7; Rosina, Marco 6; Ceci, Veronica 6; Guidobaldi, Giulio 6; Arena, Simona 8; D'Ambrosio, Chiara 8; Audano, Matteo 3
刊名	REDOX BIOLOGY
	2020-09-01
卷号	36 页码:14
关键词	Systems physiology Mitochondria Metabolism Serca Ucp1
ISSN号	2213-2317
DOI	10.1016/j.redox.2020.101633
通讯作者	Aquilano, Katia(katia.aquilano@uniroma2.it) ; Lettieri-Barbato, Daniele(daniele.lettieri.barbato@uniroma2.it)
英文摘要	Low-protein/high-carbohydrate (LPHC) diet has been suggested to promote metabolic health and longevity in adult humans and animal models. However, the complex molecular underpinnings of how LPHC diet leads to metabolic benefits remain elusive. Through a multi-layered approach, here we observed that LPHC diet promotes an energy-dissipating response consisting in the parallel recruitment of canonical and non-canonical (muscular) thermogenic systems in subcutaneous white adipose tissue (sWAT). In particular, we measured Ucp1 induction in association with up-regulation of actomyosin components and several Serca (Serca1, Serca2a, Serca2b) ATPases. In beige adipocytes, we observed that AMPK activation is responsible for transducing the amino acid lowering in an enhanced fat catabolism, which sustains both Ucp1-and Serca-dependent energy dissipation. Limiting AMPK activation counteracts the expression of brown fat and muscular genes, including Ucp1 and Serca, as well as mitochondrial oxidative genes. We observed that mitochondrial reactive oxygen species are the upstream molecules controlling AMPK-mediated metabolic rewiring in amino acid-restricted beige adipocytes. Our findings delineate a novel metabolic phenotype of responses to amino acid shortage, which recapitulates some of the benefits of cool temperature in sWAT. In conclusion, this highlights LPHC diet as a valuable and practicable strategy to prevent metabolic diseases through the enhancement of mitochondrial oxidative metabolism and the recruitment of different energy dissipating routes in beige adipocytes.
资助项目	European Foundation for the Study of Diabetes (EFSD/Lilly) ; Italian Ministry of Health[GR-2018-12367588] ; University of Rome Tor Vergata (Uncovering Excellence Program) ; AIRC[IG23562] ; National Natural Science Foundation of China[81470166] ; MIUR Progetto Eccellenza ; Italian Ministry of Health ; CNR project NUTRAGE[DSB.AD004.271]
WOS关键词	SKELETAL-MUSCLE ; CALORIC RESTRICTION ; ENERGY-EXPENDITURE ; BROWN ; MACRONUTRIENTS ; SARCOLIPIN ; ADIPOCYTES ; REGULATOR ; DISTINCT ; OBESITY
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000571102000002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291231]
专题	中国科学院上海药物研究所
通讯作者	Aquilano, Katia; Lettieri-Barbato, Daniele
作者单位	1.Univ Naples Federico II, Dept Mol Med & Med Biotechnol, Naples, Italy 2.Fdn IRCCS Casa Sollievo Sofferenza Hosp, Gastroenterol Unit, San Giovanni Rotondo, FG, Italy 3.Univ Milan, Dept Pharmacol & Biomol Sci, Milan, Italy 4.IRCCS Fdn Santa Lucia, Rome, Italy 5.Univ Molise, Dept Biosci & Terr, Pesche, IS, Italy 6.Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, Rome, Italy 7.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 8.CNR, Prote & Mass Spectrometry Lab, ISPAAM, Naples, Italy
推荐引用方式 GB/T 7714	Aquilano, Katia,Sciarretta, Francesca,Turchi, Riccardo,et al. Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue[J]. REDOX BIOLOGY,2020,36:14.
APA	Aquilano, Katia.,Sciarretta, Francesca.,Turchi, Riccardo.,Li, Bo-Han.,Rosina, Marco.,...&Lettieri-Barbato, Daniele.(2020).Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue.REDOX BIOLOGY,36,14.
MLA	Aquilano, Katia,et al."Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue".REDOX BIOLOGY 36(2020):14.