Comparative Transcriptomic and Proteomic Analyses Prove that IFN-lambda 1 is a More Potent Inducer of ISGs than IFN-alpha against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells

doi:10.1021/acs.jproteome.0c00164

	Comparative Transcriptomic and Proteomic Analyses Prove that IFN-lambda 1 is a More Potent Inducer of ISGs than IFN-alpha against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells
	Zhao, Mingzhi 1,2; Li, Liang 3; Zhai, Linhui 4; Yue, Qi 4,5; Liu, Hongyu 2; Ren, Suping 1; Jiang, Xingwei 1; Gao, Fenghua 1; Bai, Shanshan 1; Li, Honghao 6
刊名	JOURNAL OF PROTEOME RESEARCH
	2020-09-04
卷号	19 期号:9 页码:3697-3707
关键词	intestinal epithelial cell IFN-lambda 1 IFN-alpha anti-PEDV transcriptomics proteomics
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.0c00164
通讯作者	Liu, Pinghuang(liupinghuang@caas.cn) ; Tan, Minjia(mjtan@simm.ac.cn) ; Yu, Qun(yuqun1970@outlook.com)
英文摘要	Type III interferon (IFN-lambda) is currently considered to be largely nonredundant to type I interferon (IFN-alpha) in antivirus infection, especially in epithelial cells. Previous studies reported that, compared with IFN-alpha, IFN-lambda exhibited stronger induction of interferon-stimulated genes (ISGs) at the transcriptional level in intestinal epithelial cells and stronger inhibition of porcine epidemic diarrhea virus (PEDV). In this study, the different mechanisms of ISG upregulation induced by IFN-alpha and IFN-lambda 1 were compared at the mRNA and protein levels in the porcine intestinal epithelial cell model (IPEC-J2). It was proved that IFN-lambda 1 consistently exhibited stronger stimulation effects at both levels. At the mRNA level, 132 genes were significantly upregulated upon IFN-lambda 1 stimulation, while 42 genes upon IFN-alpha stimulation. At the protein level, 47 proteins were significantly upregulated upon IFN-lambda 1 stimulation, but only 8 proteins were upregulated upon IFN-alpha stimulation. The shared upregulated genes/proteins by IFN-lambda 1 in both transcriptional and translational omics, especially the regulation factors of ISG15, were involved in the JAK-STAT signaling pathway. Compared to IFN-alpha, IFN-lambda 1 could induce more consistent upregulation of the key ISGs (ISG15, USP18, OASL, and RSAD2) at 3-24 h postinduction as measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation. It was further confirmed through functional analysis that ISG15 and RSAD2 could inhibit PEDV infection in dose-dependent manners. This study provided solid evidence that IFN-lambda 1 could induce a more unique and higher ISG expression level, which exhibited anti-PEDV effects on porcine intestinal epithelial cells.
资助项目	China Postdoctoral Science Foundation[2018M633742] ; National Key R&D Program of China[2017YFC1307500] ; Innovative Research Group Project of National Natural Science Foundation of China[81821005] ; National Natural Science Foundation[81872888] ; National Natural Science Foundation[31772718] ; National Natural Science Foundation[31700724] ; Special Fund for Scientific and Technological Innovative Talents in Harbin[2017RAXYJ050] ; Innovative Research Team of High-Level Local Universities in Shanghai ; program for the 13th Five-Year Plan of China[2017ZX10202101004]
WOS关键词	INTERFERON-LAMBDA ; STRUCTURAL FEATURES ; ROTAVIRUS INFECTION ; KINETIC DIFFERENCES ; UBP43 USP18 ; III IFN ; RESPONSES ; PROTEIN ; INDUCTION ; IMMUNITY
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000569378700010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291186]
专题	中国科学院上海药物研究所
通讯作者	Liu, Pinghuang; Tan, Minjia; Yu, Qun
作者单位	1.Beijing Inst Transfus Med, Beijing 100850, Peoples R China 2.Prosit Sole Biotechnol Co Ltd, Beijing 100085, Peoples R China 3.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150069, Peoples R China 4.Chinese Acad Sci, Chem Prote Ctr, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Sartorius Sted Shanghai Trading Co Ltd, Shanghai 201210, Peoples R China 7.Peoples Liberat Army, Gen Hosp, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Mingzhi,Li, Liang,Zhai, Linhui,et al. Comparative Transcriptomic and Proteomic Analyses Prove that IFN-lambda 1 is a More Potent Inducer of ISGs than IFN-alpha against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(9):3697-3707.
APA	Zhao, Mingzhi.,Li, Liang.,Zhai, Linhui.,Yue, Qi.,Liu, Hongyu.,...&Yu, Qun.(2020).Comparative Transcriptomic and Proteomic Analyses Prove that IFN-lambda 1 is a More Potent Inducer of ISGs than IFN-alpha against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells.JOURNAL OF PROTEOME RESEARCH,19(9),3697-3707.
MLA	Zhao, Mingzhi,et al."Comparative Transcriptomic and Proteomic Analyses Prove that IFN-lambda 1 is a More Potent Inducer of ISGs than IFN-alpha against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells".JOURNAL OF PROTEOME RESEARCH 19.9(2020):3697-3707.