Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective Detection of Hg2+ in Living Cells and Zebrafish

doi:10.1021/acs.jafc.8b06895

	Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective Detection of Hg2+ in Living Cells and Zebrafish
	Gao, Tang 2; Huang, Xueyan 2; Huang, Shuai 2; Dong, Jie 3; Yuan, Kai 1; Feng, Xueping 1; Liu, Tingting 4; Yu, Kunqian 4; Zeng, Wenbin 2
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-02-27
卷号	67 期号:8 页码:2377-2383
关键词	umpolung aggregation-induced emission fluorescent probe detection of Hg2+ cell imaging zebrafish imaging
ISSN号	0021-8561
DOI	10.1021/acs.jafc.8b06895
通讯作者	Zeng, Wenbin(wbzeng@hotmail.com)
英文摘要	Using Hg2+-induced umpolung reaction and aggregation induced emission (AlE), we have rationally developed a water-soluble fluorescent probe 2,2'-(((4-(4,5-bis(4-methoxyphenyl)-1-phenyl-1H-imidazol-2-yl)phenyl)methylene)bis-(sulfanediyl))diethanol(MPIPBS) for Hg2+ detection. MPIPBS was found to have high selectivity and sensitivity toward Hg2+ detection. The mechanism of MPIPBS response to Hg2+ was verified by H-1 NMR titration, HPLC, and HAMS spectroscopy. The detection limit was examined to be 1.45 nM, which is lower than most reported probes for Hg2+. Taking advantage of excellent optical properties of AlEgen, a paper based sensor for Hg2+ detection was fabricated by immobilizing the MPIPBS on Waterman test paper. Meanwhile, MPIPBS showed satisfactory analytical performance in real water and urine samples. Further, thanks to the high water solubility, cell membrane permeability and low cytotoxicity, MPIPBS was further used to detect Hg2+ both in living cells and zebrafish. We anticipate that the prepared probe was available to detect Hg2+ in environment and biosamples.
资助项目	National Natural Science Foundation of China[81671756] ; Key Research Project of Science and Technology Foundation of Hunan Province[201751(2093)] ; State Key Laboratory of Drug Research[SIMM1803KF-14] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts041]
WOS关键词	INTRAMOLECULAR CHARGE-TRANSFER ; HEAVY-METAL IONS ; NAPHTHALENE DIIMIDE ; ORGANIC NANOPROBE ; SCHIFF-BASE ; MERCURY ; SENSOR ; CHEMOSENSOR ; CHEMODOSIMETER ; MODULATION
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000460200000031
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/290249]
专题	中国科学院上海药物研究所
通讯作者	Zeng, Wenbin
作者单位	1.Cent S Univ, Xiangya Hosp, Changsha 410078, Hunan, Peoples R China 2.Cent S Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China 3.Cent South Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Medica, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Gao, Tang,Huang, Xueyan,Huang, Shuai,et al. Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective Detection of Hg2+ in Living Cells and Zebrafish[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(8):2377-2383.
APA	Gao, Tang.,Huang, Xueyan.,Huang, Shuai.,Dong, Jie.,Yuan, Kai.,...&Zeng, Wenbin.(2019).Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective Detection of Hg2+ in Living Cells and Zebrafish.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(8),2377-2383.
MLA	Gao, Tang,et al."Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective Detection of Hg2+ in Living Cells and Zebrafish".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.8(2019):2377-2383.