Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice

doi:10.1016/j.phymed.2019.152855

	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice
	Yang, Y. Q.1,2; Wu, Y. F.3,4; Xu, F. F.1,2,5; Deng, J. B.1,2; Wu, L. L.1,2; Han, X. D.1,2,5; Liang, J.3,4; Guo, D. A.1,2,6; Liu, B.1,2,5
刊名	PHYTOMEDICINE
	2019-05-01
卷号	58 页码:12
关键词	Tripterygium wilfordii Hook F Inflammatory bowel disease apoptosis DSS T lymphocytes
ISSN号	0944-7113
DOI	10.1016/j.phymed.2019.152855
通讯作者	Liang, J.(lj102601@126.com) ; Guo, D. A.(daguo@simm.ac.cn) ; Liu, B.(doctliu@263.net)
英文摘要	Background: The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease). However, due to its severe toxicity, TG is restricted to the treatment of many diseases. Therefore, it is necessary to study a new method to obtain the attenuated and synergistic extracts from TG. Purpose: Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method. In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice. Methods: The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC). Subsequently, acute toxicity test was applied to evaluate the toxicity difference between TG and TG-n2. Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action. Results: We found that the chemical compositions of TG-n2 is different from TG. The main difference is the ratio of triptriolide (T11) / triptolide (T9). Acute toxicity test proved that TG-n2 was less toxic than TG. Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number. In addition, TG and TG-n2 can increase the apoptosis of T lymphocyte in vivo. Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells. Conclusion: This study showed that oral administration of TG-n2 is safer than TG. Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG. Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.
资助项目	National Key Research and Development Program of China[2018YFC1707900] ; National Natural Science Foundation of China[81202398] ; National Natural Science Foundation of China[81703379] ; Specific Research Project of State Administration of TCM of China[JDZX2015207] ; Guangdong Provincial Science and Technology Project[2014A020221035] ; Guangdong Provincial Science and Technology Project[2015A020211025] ; Guangdong Provincial Science and Technology Project[2015B020211013] ; Guangdong Provincial Science and Technology Project[2017B030314166] ; Guangzhou Science and Technology Project[201607010338] ; Guangzhou Science and Technology Project[201604016065] ; Special Funds for Inheriting and Developing the Traditional Chinese Medicine from TCM Bureau of Guangdong Province[20191137] ; Special Funds for Inheriting and Developing the Traditional Chinese Medicine from TCM Bureau of Guangdong Province[20191142] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2016QJ05] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2016QJ08] ; Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine[YN2015MS03] ; Key Project of High-level University Construction of Guangzhou University of Chinese Medicine[A1-AFD018181Z3961] ; Key Project of High-level University Construction of Guangzhou University of Chinese Medicine[A1-AFD018171Z11097] ; Key Project of High-level University Construction of Guangzhou University of Chinese Medicine[A1-AFD018181A34]
WOS关键词	LIVER-INJURY ; DISEASE ; STRESS ; TISSUE
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000473047100020
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289479]
专题	中国科学院上海药物研究所
通讯作者	Liang, J.; Guo, D. A.; Liu, B.
作者单位	1.Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou 510006, Guangdong, Peoples R China 2.Guangzhou Univ Chinese Med, Guangdong Prov Key Lab Clin Res Tradit Chinese Me, Guangzhou 510006, Guangdong, Peoples R China 3.Guangzhou Univ Chinese Med, Guangdong Prov Key Lab New Drug Dev & Res Chinese, Guangzhou 510006, Guangdong, Peoples R China 4.Guangzhou Univ Chinese Med, Math Engn Acad Chinese Med, Guangzhou 510006, Guangdong, Peoples R China 5.Guangdong Prov Acad Chinese Med Sci, Guangzhou 510006, Guangdong, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Y. Q.,Wu, Y. F.,Xu, F. F.,et al. Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice[J]. PHYTOMEDICINE,2019,58:12.
APA	Yang, Y. Q..,Wu, Y. F..,Xu, F. F..,Deng, J. B..,Wu, L. L..,...&Liu, B..(2019).Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.PHYTOMEDICINE,58,12.
MLA	Yang, Y. Q.,et al."Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice".PHYTOMEDICINE 58(2019):12.