Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction

doi:10.1002/advs.201802039

	Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction
	Li, Bai 1; Zhou, Xian-Hao 2,3,4; Yang, Peng-Yu 1; Zhu, Liping 1; Zhong, Yuan 1; Cai, Zhengjun 1; Jiang, Biao 3,4; Cai, Xiaoqing 1; Liu, Jia 3; Jiang, Xianxing 1
刊名	ADVANCED SCIENCE
	2019-07-03
卷号	6 期号:13 页码:11
关键词	fluorogenic labeling nitroso-diene cycloaddition photoactivatable protein imaging
DOI	10.1002/advs.201802039
通讯作者	Liu, Jia(liujia@shanghaitech.edu.cn) ; Jiang, Xianxing(jiangxx5@mail.sysu.edu.cn)
英文摘要	Fluorogenic labeling enables imaging cellular molecules of interest with minimal background. This process is accompanied with the notable increase of the quantum yield of fluorophore, thus minimizing the background signals from unactivated profluorophores. Herein, the development of a highly efficient and bioorthogonal nitroso-based Diels-Alder fluorogenic reaction is presented and its usefulness is validated as effective and controllable in fluorescent probes and live-cell labeling strategies for dynamic cellular imaging. It is demonstrated that nitroso-based cycloaddition is an efficient fluorogenic labeling tool through experiments of further UV-activatable fluorescent labeling on proteins and live cells. The ability of tuning the fluorescence of labeled proteins by UV-irradiation enables selective activation of proteins of interest in a particular cell compartment at a given time point, while leaving the remaining labeled molecules untouched.
资助项目	Major Research Program of National Natural Science Foundation of China[91853106] ; Program for Guangdong Introducing Innovative and Enterpreneurial Teams[2016ZT06Y337] ; ShanghaiTech University[2019F0301-000-01] ; Thousand Young Talents Program
WOS关键词	INDEPENDENT CHEMICAL-SHIFTS ; LIVE-CELL ; TETRAZINE PROBES ; IN-VIVO ; LIGATION ; CYCLOADDITION ; FLUORESCENCE ; TECHNOLOGY ; CHEMISTRY
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	WILEY
WOS记录号	WOS:000477710600006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/289100]
专题	中国科学院上海药物研究所
通讯作者	Liu, Jia; Jiang, Xianxing
作者单位	1.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Bai,Zhou, Xian-Hao,Yang, Peng-Yu,et al. Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction[J]. ADVANCED SCIENCE,2019,6(13):11.
APA	Li, Bai.,Zhou, Xian-Hao.,Yang, Peng-Yu.,Zhu, Liping.,Zhong, Yuan.,...&Jiang, Xianxing.(2019).Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction.ADVANCED SCIENCE,6(13),11.
MLA	Li, Bai,et al."Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction".ADVANCED SCIENCE 6.13(2019):11.