microrna1304suppresseshumannonsmallcelllungcancercellgrowthinvitrobytargetinghemeoxygenase1

doi:10.1038/aps.2016.92

	microrna1304suppresseshumannonsmallcelllungcancercellgrowthinvitrobytargetinghemeoxygenase1
	Li Chenggang 1; Pu Mengfan 1; Li Chunzhu 1; Gao Man 1; Liu Mingxia 1; Yu Cunzhi 1; Yan Hong 1; Peng Chun 1; Zhao Yang 1; Li Yu 1
刊名	actapharmacologicasinica
	2017
卷号	38 期号:1 页码:110
关键词	miR-1304 NSCLC heme oxygenase-1 hemin
ISSN号	1671-4083
DOI	10.1038/aps.2016.92
英文摘要	Previous studies have shown that microRNA-1304 (miR-1304) is dysregulated in certain types of cancers, including non-small cell lung cancer (NSCLC), and might be involved in tumor survival and/or growth. In this study we investigated the direct target of miR-1304 and its function in NSCLC in vitro. Human lung adenocarcinoma cell lines (A549 and NCI-H1975) were studied. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays. Cell apoptosis and cell cycle were examined using annexin V-PE/7-AAD and PI staining assays, respectively. The dual-luciferase reporter assay was used to verify post-transcriptional regulation of heme oxygenase-1 (HO-1) by miR-1304. CRISPR/Cas9 was used to deplete endogenous miR-1304. Overexpression of MiR-1304 significantly decreased the number and viability of NSCLC cells and colony formation, and induced cell apoptosis and G_0/ G_1 phase cell cycle arrest. HO-1 was demonstrated to be a direct target of miR-1304 in NSCLC cells. Restoration of HO-1 expression by hemin (20 μmol/L) abolished the inhibition of miR-1304 on cell growth and rescued miR-1304-induced apoptosis in A549 cells. Suppression of endogenous miR-1304 with anti-1304 significantly increased HO-1 expression and promoted cell growth and survival in A549 cells. In 17 human NSCLC tissue samples, miR-1304 expression was significantly decreased, while HO-1 expression was significantly increased as compared to normal lung tissues. MicroRNA-1304 is a tumor suppressor and HO-1 is its direct target in NSCLC. The results suggest the potential for miR-1304 as a therapeutic target for NSCLC.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/283984]
专题	中国科学院上海药物研究所
作者单位	1.中国科学院上海药物研究所 2.The Brain Science Center, Beijing Institute of Basic Medical Sciences
推荐引用方式 GB/T 7714	Li Chenggang,Pu Mengfan,Li Chunzhu,et al. microrna1304suppresseshumannonsmallcelllungcancercellgrowthinvitrobytargetinghemeoxygenase1[J]. actapharmacologicasinica,2017,38(1):110.
APA	Li Chenggang.,Pu Mengfan.,Li Chunzhu.,Gao Man.,Liu Mingxia.,...&Ren Jin.(2017).microrna1304suppresseshumannonsmallcelllungcancercellgrowthinvitrobytargetinghemeoxygenase1.actapharmacologicasinica,38(1),110.
MLA	Li Chenggang,et al."microrna1304suppresseshumannonsmallcelllungcancercellgrowthinvitrobytargetinghemeoxygenase1".actapharmacologicasinica 38.1(2017):110.