agglutininisolatedfromarisemaheterophyllumblumeinducesapoptosisandautophagyina549cellsthroughinhibitingpi3kaktpathwayandinducingerstress

	agglutininisolatedfromarisemaheterophyllumblumeinducesapoptosisandautophagyina549cellsthroughinhibitingpi3kaktpathwayandinducingerstress
	Feng Lixing; Sun Peng; Mi Tian; Liu Miao; Liu Wang; Yao Si; Cao Yimin; Yu Xiaolu; Wu Wanying; Jiang Baohong
刊名	chinesejournalofnaturalmedicines
	2016
卷号	014 期号:011 页码:856
关键词	Agglutinin Arisaema heterophyllum Blume Human non-small-cell lung cancer A549 cell line Apoptosis Autophagy
ISSN号	2095-6975
英文摘要	Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis （RA）. RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the anderlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume （AHA） and explore the possible mechanisms in human non-small-cell lung cancer A549 zells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction zhromatography. AHA dose-dependently inhibited the proliferation of A549 cells and induced G~ phase cell cycle arrest. AHA induced apoptosis by up-regulating pro-apoptotic Bax, decreasing anti-apoptotic Bcl-2, and activating caspase-9 and caspase-3. In A549 cells treated with AHA, the PI3K/Akt pathway was inhibited. Furthermore, AHA induced increase in the levels of ER stress markers such as phosphorylated eukaryotic initiation factor 2a （p-eIF2a）, C/EBP-homologous protein （CHOP）, inositol-requiring enzyme l a （IREla）, and phosphorylated c-Jun NH2-terminal kinase （p-JNK）. AHA also induced autophagy in A549 cells. Staining of acidic vesicular organelles ＇,AVOs） and increase in the levels of LC3II and ATG7 were observed in AHA-treated cells. These findings suggested that AHA might be one of the active components with anti-cancer effects in Arisaema heterophyllum Blume. In conclusion, cytotoxicity of AHA on cancer zells might be related to its effects on apoptosis and autophagy through inhibition of PI3K/Akt pathway and induction of ER stress.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/282873]
专题	中国科学院上海药物研究所
作者单位	中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Feng Lixing,Sun Peng,Mi Tian,et al. agglutininisolatedfromarisemaheterophyllumblumeinducesapoptosisandautophagyina549cellsthroughinhibitingpi3kaktpathwayandinducingerstress[J]. chinesejournalofnaturalmedicines,2016,014(011):856.
APA	Feng Lixing.,Sun Peng.,Mi Tian.,Liu Miao.,Liu Wang.,...&Liu Xuan.(2016).agglutininisolatedfromarisemaheterophyllumblumeinducesapoptosisandautophagyina549cellsthroughinhibitingpi3kaktpathwayandinducingerstress.chinesejournalofnaturalmedicines,014(011),856.
MLA	Feng Lixing,et al."agglutininisolatedfromarisemaheterophyllumblumeinducesapoptosisandautophagyina549cellsthroughinhibitingpi3kaktpathwayandinducingerstress".chinesejournalofnaturalmedicines 014.011(2016):856.