Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst

doi:10.1016/j.ijbiomac.2019.09.046

	Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst
	Fu, Youli 3; Shi, Lei 1,2,3; Ding, Kan 2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-12-01
卷号	141 页码:693-699
关键词	Polysaccharide Anti-tumor activity beta-Glucan Structure Ganoderma lucidum
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.09.046
通讯作者	Shi, Lei(shilei@tp.edu.sg)
英文摘要	To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.) Karst. The average molecular weight of this polysaccharide was estimated to be 1.5 x 10(4) Da. Monosaccharide composition analysis indicated that it was composed solely of glucose. From methylation analysis, H-1 and C-13 NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained beta-D-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 -> 3)-beta-D-glucopyranosyl residues, to which the side chain consisting of terminal and (1 -> 6)-beta-D-glucopyranosyl residue is attached at position O-6 of the branching residues. Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice. Furthermore, no drug-related toxic reactions were observed. It was suggested that WGLP might be a potential anti-tumor agent used in the clinic. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China (NSFC)[30970640] ; Knowledge Innovation Program of the Chinese Academy of Sciences[SIMM0912QN-10]
WOS关键词	RETICULOENDOTHELIAL SYSTEM ; NEUTRAL POLYSACCHARIDE ; ANTIDIABETES DRUGS ; EDIBLE MUSHROOM ; GLUCAN ; PURIFICATION ; CELL ; CYTOKINES ; FEATURES ; GLYCANS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000506713000072
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/282494]
专题	中国科学院上海药物研究所
通讯作者	Shi, Lei
作者单位	1.Temasek Polytech, Sch ofApplied Sci, Singapore 529757, Singapore 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Qufu Normal Univ, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Fu, Youli,Shi, Lei,Ding, Kan. Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,141:693-699.
APA	Fu, Youli,Shi, Lei,&Ding, Kan.(2019).Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,141,693-699.
MLA	Fu, Youli,et al."Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 141(2019):693-699.