On-Line Electrokinetic Supercharging and Sweeping for the Preconcentration and Determination of Nucleosides and Related Compounds by Capillary Electrophoresis

doi:10.1080/00032719.2020.1725033

	On-Line Electrokinetic Supercharging and Sweeping for the Preconcentration and Determination of Nucleosides and Related Compounds by Capillary Electrophoresis
	Li, Wenjia 1,3; Qian, Zhengming 3; Li, Chunhong 3; Guo, Dean 2; Wei, Jiangchun 1; Liu, Xingzhong 1
刊名	ANALYTICAL LETTERS
	2020-02-08
页码	13
关键词	Chinese cordyceps electrokinetic supercharging micellar electrokinetic chromatography nucleobases nucleosides sweeping
ISSN号	0003-2719
DOI	10.1080/00032719.2020.1725033
通讯作者	Liu, Xingzhong(liuxz@im.ac.cn)
英文摘要	Nucleosides and related compounds have vital physiological and pharmacological activities. In this study, on-line preconcentration using electrokinetic supercharging (EKS)-sweeping method was proposed to enhance the efficiency of the capillary electrophoresis separation of the nucleosides and related compounds. The separation conditions, including the pH of the borate buffer (terminating electrolyte), the concentration and pH of the phosphate buffer (leading electrolyte), the concentration of sodium dodecyl sulfate (SDS) and the electrokinetic injection (EKI) time, were investigated in detail. The optimum EKS-sweeping conditions were as follows: 40 mM sodium phosphate buffer at pH 2.0 containing 50 mM SDS as the BGE, 120 mM sodium phosphate buffer at pH 2.0 as the leading electrolyte, 30 mM borate buffer at pH 12.0 as the terminating electrolyte, and EKI of the sample at -10 kV for 30 s. The separation was performed by micellar electrokinetic chromatography at -5 kV. Under these conditions, the sensitivity values of five analytes were enhanced from 26 to 109-fold compared to a standard capillary zone electrophoresis (CZE) procedure. The intra- and inter-day repeatability values (as the relative standard deviation) were less than 1.1% and 5.2% for the migration time and lower than 4.4% and 4.8% for the peak areas. Finally, the developed method was successfully applied for the determination of nucleosides and related compounds in Chinese cordyceps samples.
资助项目	National Key R&D Program of China[2018YFC1706101] ; Dongguan Academician Workstation Project[DGYSZ-2018-03]
WOS关键词	ZONE-ELECTROPHORESIS ; CULTURED CORDYCEPS ; MASS-SPECTROMETRY ; SAMPLE STACKING ; 2-STEP STACKING ; NUCLEOBASES ; MICELLE
WOS研究方向	Chemistry
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000513368500001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281700]
专题	中国科学院上海药物研究所
通讯作者	Liu, Xingzhong
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Guangdong, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai, Peoples R China 3.Sunshine Lake Pharma Co, Key Lab State Adm Tradit Chinese Med, Dongguan, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenjia,Qian, Zhengming,Li, Chunhong,et al. On-Line Electrokinetic Supercharging and Sweeping for the Preconcentration and Determination of Nucleosides and Related Compounds by Capillary Electrophoresis[J]. ANALYTICAL LETTERS,2020:13.
APA	Li, Wenjia,Qian, Zhengming,Li, Chunhong,Guo, Dean,Wei, Jiangchun,&Liu, Xingzhong.(2020).On-Line Electrokinetic Supercharging and Sweeping for the Preconcentration and Determination of Nucleosides and Related Compounds by Capillary Electrophoresis.ANALYTICAL LETTERS,13.
MLA	Li, Wenjia,et al."On-Line Electrokinetic Supercharging and Sweeping for the Preconcentration and Determination of Nucleosides and Related Compounds by Capillary Electrophoresis".ANALYTICAL LETTERS (2020):13.