A Trojan horse biomimetic delivery strategy using mesenchymal stem cells for PDT/PTT therapy against lung melanoma metastasis

doi:10.1039/c9bm01401b

	A Trojan horse biomimetic delivery strategy using mesenchymal stem cells for PDT/PTT therapy against lung melanoma metastasis
	Ouyang, Xumei 1,2,3,4; Wang, Xiaoling 1,2,3; Kraatz, Heinz-Bernhard 5; Ahmadi, Soha 5; Gao, Jianqing 1,3; Lv, Yuanyuan 2; Sun, Xiaoyi 2; Huang, Yongzhuo 6
刊名	BIOMATERIALS SCIENCE
	2020-02-21
卷号	8 期号:4 页码:1160-1170
ISSN号	2047-4830
DOI	10.1039/c9bm01401b
通讯作者	Sun, Xiaoyi(sunxiaoyi@zucc.edu.cn) ; Huang, Yongzhuo(yzhuang@simm.ac.cn)
英文摘要	Mesenchymal stem cell (MSC)-based biomimetic delivery has been actively explored for drug accumulation and penetration into tumors by taking advantage of the tumor-tropic and penetration properties of MSCs. In this work, we further demonstrated the feasibility of MSC-mediated nano drug delivery, which was characterized by the "Trojan horse"-like transport via an endocytosis-exocytosis-endocytosis process between MSCs and cancer cells. Chlorin e6 (Ce6)-conjugated polydopamine nanoparticles (PDA-Ce6) were developed and loaded into the MSCs. Phototherapeutic agents are safe to the MSCs, and their very low dark toxicity causes no impairment of the inherent properties of MSCs, including tumor-homing ability. The MSCs loaded with PDA-Ce6 (MSC-PDA-Ce6) were able to target and penetrate into tumors and exocytose 60% of the payloads in 72 h. The released PDA-Ce6 NPs could penetrate deep and be re-endocytosed by the cancer cells. MSC-PDA-Ce6 tended to accumulate in the lungs and delivered PDA-Ce6 into the tumors after intravenous injection in the mouse model with lung melanoma metastasis. Phototoxicity can be selectively triggered in the tumors by sequentially treating with near-infrared irradiation to induce photodynamic therapy (PDT) and photothermal therapy (PTT). The MSC-based biomimetic delivery of PDA-Ce6 nanoparticles is a potential method for dual phototherapy against lung melanoma metastasis.
资助项目	National Natural Science Foundation of China[81925035] ; National Natural Science Foundation of China[81402872] ; National Natural Science Foundation of China[81620108028] ; Natural Science Foundation of Zhejiang Province[LY17H160002] ; Natural Science Foundation of Zhejiang Province[LY17B020002] ; Agricultural and Social Development Program of Hangzhou[20191203B48]
WOS关键词	LOADED NANOPARTICLES ; PHOTOTHERMAL THERAPY ; CELLULAR UPTAKE ; GENE DELIVERY ; CANCER ; EXOCYTOSIS ; NANOCARRIERS ; ENHANCEMENT ; PENETRATION ; VEHICLE
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000517148800011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/281403]
专题	中国科学院上海药物研究所
通讯作者	Sun, Xiaoyi; Huang, Yongzhuo
作者单位	1.Wenzhou Med Univ, Eye Hosp, Wenzhou 325035, Peoples R China 2.Zhejiang Univ City Coll, Dept Pharm, Hangzhou 310015, Peoples R China 3.Wenzhou Med Univ, Sch Ophthalmol & Optometry, Dept Pharm, Wenzhou 325035, Peoples R China 4.Zhejiang Univ, Coll Pharmaceut Sci, Dept Pharmaceut, Hangzhou 310058, Peoples R China 5.Univ Toronto, Dept Phys & Environm Sci, Scarborough, ON M1C 1A4, Canada 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Ouyang, Xumei,Wang, Xiaoling,Kraatz, Heinz-Bernhard,et al. A Trojan horse biomimetic delivery strategy using mesenchymal stem cells for PDT/PTT therapy against lung melanoma metastasis[J]. BIOMATERIALS SCIENCE,2020,8(4):1160-1170.
APA	Ouyang, Xumei.,Wang, Xiaoling.,Kraatz, Heinz-Bernhard.,Ahmadi, Soha.,Gao, Jianqing.,...&Huang, Yongzhuo.(2020).A Trojan horse biomimetic delivery strategy using mesenchymal stem cells for PDT/PTT therapy against lung melanoma metastasis.BIOMATERIALS SCIENCE,8(4),1160-1170.
MLA	Ouyang, Xumei,et al."A Trojan horse biomimetic delivery strategy using mesenchymal stem cells for PDT/PTT therapy against lung melanoma metastasis".BIOMATERIALS SCIENCE 8.4(2020):1160-1170.