CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment

doi:10.1038/s41401-019-0322-9

	CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment
	Xing, Hui 1,2; Meng, Ling-hua 1,2
刊名	ACTA PHARMACOLOGICA SINICA
	2020-05-01
卷号	41 期号:5 页码:583-587
关键词	precision medicine CRISPR-cas9 drug target biomarker drug resistance synergistic lethality
ISSN号	1671-4083
DOI	10.1038/s41401-019-0322-9
通讯作者	Meng, Ling-hua(lhmeng@simm.ac.cn)
英文摘要	Cancer is a highly heterogeneous disease in term of molecular signature even though it is originated from the same tissue type. Cancer heterogeneity may occur during its development or treatment, which is the main cause resulting in drug resistance and recurrence. Precision medicine refers to matching the right medicine to the right patients based on their molecular signatures. Therefore, a thorough understanding of the mechanism of tumorigenesis and drug resistance is essential to precision medicine. CRISPR-cas9 system is a powerful tool for gene editing and CRISPR-based high-throughput screening has been widely applied especially in searching for tumor-driven or synergistic lethal genes aiming to overcome drug resistance. In this review, we describe the progress of CRISPR-cas9-based unbiased screening in precision medicine including identification of new drug targets, biomarkers and elucidation of mechanisms leading to drug resistance. The existing challenges as well as the future directions are also discussed.
资助项目	Chinese Academy of Sciences[XDA12020111] ; National Science and Technology Major Project Key New Drug Creation and Manufacturing Program[2018ZX09711002-011-014] ; National Science and Technology Major Project Key New Drug Creation and Manufacturing Program[2018ZX09711002-004-011] ; National Natural Science Foundation of China[81773760] ; Fudan-SIMM Joint Research Fund[FU-SIMM20172005]
WOS关键词	RNA ; CAS ; DISCOVERY ; SCREENS ; CRISPR/CAS9 ; GENERATION ; EVOLUTION ; TARGETS ; DESIGN ; GUIDE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000529703000001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280558]
专题	中国科学院上海药物研究所
通讯作者	Meng, Ling-hua
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Xing, Hui,Meng, Ling-hua. CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment[J]. ACTA PHARMACOLOGICA SINICA,2020,41(5):583-587.
APA	Xing, Hui,&Meng, Ling-hua.(2020).CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment.ACTA PHARMACOLOGICA SINICA,41(5),583-587.
MLA	Xing, Hui,et al."CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment".ACTA PHARMACOLOGICA SINICA 41.5(2020):583-587.