Structure of formylpeptide receptor 2-G i complex reveals insights into ligand recognition and signaling

doi:10.1038/s41467-020-14728-9

	Structure of formylpeptide receptor 2-G i complex reveals insights into ligand recognition and signaling
	Youwen Zhuang ; Heng Liu ; X. Edward Zhou ; Ravi Kumar Verma ; Parker W. de Waal ; Wonjo Jang ; Ting-Hai Xu ; Lei Wang ; Xing Meng ; Gongpu Zhao
刊名	naturecommunications
	2020
卷号	11 期号:1 页码:1
ISSN号	2041-1723
DOI	10.1038/s41467-020-14728-9
英文摘要	Formylpeptide receptors (FPRs) as G protein-coupled receptors (GPCRs) can recognize formylpeptides derived from pathogens or host cells to function in host defense and cell clearance. In addition, FPRs, especially FPR2, can also recognize other ligands with a large chemical diversity generated at different stages of inflammation to either promote or resolve inflammation in order to maintain a balanced inflammatory response. The mechanism underlying promiscuous ligand recognition and activation of FPRs is not clear. Here we report a cryo-EM structure of FPR2-G i signaling complex with a peptide agonist. The structure reveals a widely open extracellular region with an amphiphilic environment for ligand binding. Together with computational docking and simulation, the structure suggests a molecular basis for the recognition of formylpeptides and a potential mechanism of receptor activation, and reveals conserved and divergent features in G i coupling. Our results provide a basis for understanding the molecular mechanism ofthe functional promiscuity of FPRs.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/280397]
专题	中国科学院上海药物研究所
作者单位	1.University of Pittsburgh 2.Takeda Research 3.University of Chinese Academy of Sciences 4.Center for Cancer and Cell Biology, Program for Structural Biology, Van Andel Research Institute 5.David Van Andel Advanced Cryo-Electron Microscopy Suite, Van Andel Research Institute 6.Medical College of Georgia, Augusta University 7.Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR) 8.The CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Youwen Zhuang,Heng Liu,X. Edward Zhou,et al. Structure of formylpeptide receptor 2-G i complex reveals insights into ligand recognition and signaling[J]. naturecommunications,2020,11(1):1.
APA	Youwen Zhuang.,Heng Liu.,X. Edward Zhou.,Ravi Kumar Verma.,Parker W. de Waal.,...&Cheng Zhang.(2020).Structure of formylpeptide receptor 2-G i complex reveals insights into ligand recognition and signaling.naturecommunications,11(1),1.
MLA	Youwen Zhuang,et al."Structure of formylpeptide receptor 2-G i complex reveals insights into ligand recognition and signaling".naturecommunications 11.1(2020):1.