Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro

doi:10.1021/acsbiomaterials.8b01000

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	Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro
	Chen, Jie 1,3; Li, Jiongjiong 1,3; Hu, Fu 1,3; Zou, Qin 1,3; Mei, Quanjing 1,3; Li, Shujun 2; Hao, Yulin 2; Hou, Wentao 2; Li, Jidong 1,3; Li, Yubao 1,3
刊名	ACS BIOMATERIALS SCIENCE & ENGINEERING
	2019-02-01
卷号	5 期号:2 页码:572-581
关键词	titanium alloy scaffold EBM-MAO strategy affinity surface stereoscopic cytoskeleton structure LIPUS stimulation cell mineralization
ISSN号	2373-9878
DOI	10.1021/acsbiomaterials.8b01000
通讯作者	Zuo, Yi(zoae@vip.sina.com)
英文摘要	The porous Ti6Al4V alloy has emerged to solve the biomechanical mismatch between implant and bone as its tunable mechanical properties. Cell-surface interaction is related to numerous factors-the surface's chemical composition, morphological structure, and external effect. The microarc oxidation (MAO) method was employed in this study to improve the surface properties of scaffolds produced by Electron Beam Melting (EBM), and low-intensity pulse ultrasound (LIPUS) provides physical stimulation for cells in vitro. Although MAO-treated and untreated scaffolds shared the same three-dimensional (3D) structures, the former recreated a more affinity surface than the latter in the 3D room, which could stimulate cell adhesion, proliferation, and differentiation. Therefore, MG63 cells were represented with a stereoscopic cytoskeleton structure on the MAO-treated scaffold as numerous cellular filopodia/lamellipodia with rich extracellular matrix secretion, while flat and sheetlike cells were observed on the untreated scaffold. The expression of ALP, OCN, BMP2, Bmpr1a, and Runx2 were up-regulated by the MAO-treated scaffold; in addition, LIPUS stimulation effectively promoted cell proliferation and osteogenesis differentiation. In the future, the EBM-MAO strategy can be applied to prepare 3D hierarchical macro-/microstructure titanium implants for bone grafts, and LIPUS stimulation can be used as a therapeutic method simultaneously.
资助项目	China NSFC Project[31370971] ; Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-JSC031-02]
WOS研究方向	Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000458937900019
资助机构	China NSFC Project ; Key Research Program of Frontier Sciences, CAS
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/131902]
专题	金属研究所_中国科学院金属研究所
通讯作者	Zuo, Yi
作者单位	1.Sichuan Univ, Analyt & Testing Ctr, Chengdu 610064, Sichuan, Peoples R China 2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China 3.Sichuan Univ, Res Ctr Nano Biomat, Chengdu 610064, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jie,Li, Jiongjiong,Hu, Fu,et al. Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2019,5(2):572-581.
APA	Chen, Jie.,Li, Jiongjiong.,Hu, Fu.,Zou, Qin.,Mei, Quanjing.,...&Zuo, Yi.(2019).Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro.ACS BIOMATERIALS SCIENCE & ENGINEERING,5(2),572-581.
MLA	Chen, Jie,et al."Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro".ACS BIOMATERIALS SCIENCE & ENGINEERING 5.2(2019):572-581.