Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28

doi:10.1080/09168451.2018.1490170

CORC > 金属研究所 > 中国科学院金属研究所

	Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28
	Cao, Ting 1,2; Ling, Junhong 3; Liu, Yi 1,3; Chen, Xiaoqi 1,2; Tian, Xiaoyue 1,3; Meng, Dali 3; Pan, Huaqi 1; Hu, Jiangchun 1; Wang, Nan 1
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2018
卷号	82 期号:10 页码:1832-1839
关键词	Cyclopiazonic acid Aspergillus oryzae oxidative burst hybrid pks-nrps plant immune
ISSN号	0916-8451
DOI	10.1080/09168451.2018.1490170
通讯作者	Wang, Nan(wangn@iae.ac.cn)
英文摘要	Extracellular alkalinization and H2O2 production are important early events during induced resistance establishment in plants. In a screen for metabolites as plant resistance activators from 98 fungal isolates associated with marine sponge Hymeniacidon perleve, the cyclopiazonic acids (CPAs) produced by Aspergillus oryzae HMP-F28 induced significant extracellular alkalinization coupled with augmented H2O2 production in tobacco cell suspensions. Bioassay-guided fractionation led to the isolation and structural elucidation of a new CPA congener (4, 3-hydroxysperadine A) and three known ones (1-3). To construct a mutasynthetic strain to generate unnatural CPA analogues, a hybrid pks-nrps gene (cpaS) was disrupted to abolish the production of the critical precursor of cyclo-acetoacetyl-L-tryptophan (cAATrp) and all the downstream CPA products. Elimination of cAATrp will allow cAATrp mimics being processed by the CPA biosynthetic machinery to produce CPA derivatives with designed structural features.
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000445240200020
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/129433]
专题	金属研究所_中国科学院金属研究所
通讯作者	Wang, Nan
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Cao, Ting,Ling, Junhong,Liu, Yi,et al. Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2018,82(10):1832-1839.
APA	Cao, Ting.,Ling, Junhong.,Liu, Yi.,Chen, Xiaoqi.,Tian, Xiaoyue.,...&Wang, Nan.(2018).Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,82(10),1832-1839.
MLA	Cao, Ting,et al."Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 82.10(2018):1832-1839.