Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process

doi:10.2147/IJN.SI46866

CORC > 金属研究所 > 中国科学院金属研究所

	Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process
	Wang, Lei 2; Li, Guoyuan 2; Ren, Ling 3; Kong, Xiangdong 2; Wang, Yugang 2; Han, Xiuguo 2; Jiang, Wenbo 1; Dai, Kerong 2; Yang, Ke 3; Hao, Yongqiang 2
刊名	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2017
卷号	12 页码:8443-8457
关键词	nano-sized copper lysyl oxidise osteogenesis fracture healing callus evolution
ISSN号	1178-2013
DOI	10.2147/IJN.SI46866
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Hao, Yongqiang(hyq_9hospital@hotmail.com)
英文摘要	Treatment for fractures requires internal fixation devices, which are mainly produced from stainless steel or titanium alloy without biological functions. Therefore, we developed a novel nano-copper-bearing stainless steel with nano-sized copper-precipitation (317L-Cu SS). Based on previous studies, this work explores the effect of 317L-Cu SS on fracture healing; that is, proliferation, osteogenic differentiation, osteogenesis-related gene expression, and lysyl oxidase activity of human bone mesenchymal stem cells were detected in vitro. Sprague Dawley rats were used to build an animal fracture model, and fracture healing and callus evolution were investigated by radiology (X-ray and micro-CT), histology (II&E, Masson, and safranin O/fast green staining), and histomorphometry. Further, the Cu2+ content and It unx2 level in the callus were determined, and local mechanical test of the fracture was performed to assess the healing quality. Our results revealed that 317L-Cu SS did not affect the proliferation of human bone mesenchymal stem cells, but promoted osteogenic differentiation and the expression of osteogenesis-related genes. In addition, 317L-Cu SS upregulated the lysyl oxidase activity. The X-ray and micro-CT results showed that the callus evolution efficiency and fracture healing speed were superior for 317L-Cu SS. Histological staining displayed large amounts of fibrous tissues at 3 weeks, and cartilage and new bone at 6 weeks. Further, histomorphometric analysis indicated that the callus possessed higher osteogenic efficiency at 6 weeks, and a high Cu2+ content and increased Runx2 expression were observed in the callus for 317L-Cu SS. Besides, the mechanical strength of the fracture site Was much hotter than that of the control group. Overall, we conclude that 317L-Cu SS possesses the ability to increase Cu2+ content and promote osteogenesis in the callus, which could accelerate the callus evolution process and bone formation to provide faster and better fracture healing.
资助项目	National Key Research and Development Program of China[2016YFC1100600] ; National Natural Science Foundation of China[81071472] ; National Natural Science Foundation of China[81371960] ; National Natural Science Foundation of China[51631009] ; Key National Basic Research Program of China[2012CB619101] ; Technology Support Project of the Science and Technology Commission of Shanghai[13441901302] ; Technology Support Project of the Science and Technology Commission of Shanghai[14441901000] ; Technology Support Project of the Science and Technology Commission of Shanghai[15411951200] ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai[SHDC12014124] ; Shanghai Jiao Tong University Cross Research Fund of Medical Engineering[YG2013MS57] ; Multicenter Clinical Research Project of Shanghai Jiao Tong University School of Medicine[DLY201506] ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine[BXJ201429]
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
语种	英语
出版者	DOVE MEDICAL PRESS LTD
WOS记录号	WOS:000416144200001
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Key National Basic Research Program of China ; Technology Support Project of the Science and Technology Commission of Shanghai ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai ; Shanghai Jiao Tong University Cross Research Fund of Medical Engineering ; Multicenter Clinical Research Project of Shanghai Jiao Tong University School of Medicine ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/127095]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yang, Ke; Hao, Yongqiang
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Med Printing Innovat Res Ctr 3D, Shanghai, Peoples R China 2.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed, Shanghai Key Lab Orthopaed Implants,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Special Mat & Device Res Dept, Shenyang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lei,Li, Guoyuan,Ren, Ling,et al. Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12:8443-8457.
APA	Wang, Lei.,Li, Guoyuan.,Ren, Ling.,Kong, Xiangdong.,Wang, Yugang.,...&Hao, Yongqiang.(2017).Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process.INTERNATIONAL JOURNAL OF NANOMEDICINE,12,8443-8457.
MLA	Wang, Lei,et al."Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process".INTERNATIONAL JOURNAL OF NANOMEDICINE 12(2017):8443-8457.