Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell
	Jianlong Ma ; Xiaofeng Lu ; Honglin Zhai ; Qiang Li ; Li Qiao ; Yong Guo
刊名	Talanta
	2020
期号	219 页码:121278
英文摘要	In the current work, a near-infrared (NIR) fluorescent probe (CyClCP) was developed for fast (35 min), highly sensitive (LOD of 3.75 U/L) and selective response to BChE in vitro and in vivo. Upon the addition of BChE, CyClCP could be efficiently activated with remarkable NIR (λem= 708 nm) fluorescence enhancement and obvious absorbance red shift (581 nm–687 nm). Specifically, according to the subtle differences structural features and substrate preference between BChE and its sister enzyme AChE, CyClCP was constructed by in- troducing chlorine atom at the ortho-position of the phenolic hydroxyl in the previous reported probe (CyCP). Fortunately, CyClCP exhibited better selectivity towards BChE over AChE compared with CyCP. This molecular design strategy was further rationalized by docking molecular of fluorescence probes (CyClCP and CyCP) and enzymes (BChE and AChE). Finally, CyClCP was membrane permeable and successfully applied to image en- dogenous BChE level in HepG2 and LO2 cells. Therefore, CyClCP could serve as a promising tool for BChE- related physiological function studies in complex biological systems.
内容类型	期刊论文
源URL	[http://ir.licp.cn/handle/362003/26569]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Li Qiao; Yong Guo
作者单位	1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, 2.College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China 3.University of Chinese Academy of Sciences, Beijing, 100049, PR China 4.Department of Neurosurgery, Gansu Provincial Hospital, Lanzhou, 730000, PR China 5.ey Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou,
推荐引用方式 GB/T 7714	Jianlong Ma,Xiaofeng Lu,Honglin Zhai,et al. Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell[J]. Talanta,2020(219):121278.
APA	Jianlong Ma,Xiaofeng Lu,Honglin Zhai,Qiang Li,Li Qiao,&Yong Guo.(2020).Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell.Talanta(219),121278.
MLA	Jianlong Ma,et al."Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell".Talanta .219(2020):121278.