Global microRNA and isomiR expression associated with liver metabolism is induced by organophosphorus flame retardant exposure in male Chinese rare minnow (Gobiocypris rarus)

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Global microRNA and isomiR expression associated with liver metabolism is induced by organophosphorus flame retardant exposure in male Chinese rare minnow (Gobiocypris rarus)
	Hong, Xiangsheng ; Chen, Rui ; Yuan, Lilai ; Zha, Jinmiao
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-02-01
卷号	649 页码:829-838
关键词	Organophosphorus flame retardants (OPFRs) microRNA isomiR Bioinformatics Liver metabolism Chinese rare minnow (Gobiocypris rants)
ISSN号	0048-9697
英文摘要	To reveal the adverse effects of organophosphorus flame retardants (OPFRs) on aquatic organisms at the epigenetic level, male Chinese rare minnows were exposed to 0.24 mg/L tris(2 butoxyethyl) phosphate (TBOEP), 0.04 mg/L tris(1,3 dichloro 2 propyl) phosphate (TDCIPP), or 0.012 mg/L triphenyl phosphate (TPHP) for 14 days. The effects of sub-acute OPFR exposure on liver miRNA and the 3' isomiR expression profiles of Chinese rare minnows were investigated. Through small RNA sequencing and bioinformatics analysis, a total of 32, 84, and 19 differentially expressed miRNAs were detected for TBOEP, TDCIPP, and TPHP exposure, respectively (p < 0.05). Target prediction of the differentially expressed miRNAs and pathway enrichment analysis indicated that predicted altered mRNAs for all three OPFRs were associated with metabolic pathways, whereas base excision repair was only predicted to be perturbed by the TPHP treatment. In addition, 3' isomiR-Us were unexpectedly abundant in all groups (e.g., miR-143). and TDCIPP strongly increased the ratio of 3' isomiR-U expression. Finally, histological examination and metabolic enzyme activity analyses werein agreement with the predicted metabolic pathways. As such, our study indicates that the investigation of epigenetics changes in miRNA gene transcription is a considerable method for the assessment of aquatic toxicity. (C) 2018 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43471]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Ind Wastewater Treatment & Reuse, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 4.Chinese Acad Sci, Key Lab Drinking Water Sci & Technol, Res Ctr Ecoenvironm Sci, 18 Shuangqing Rd, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Hong, Xiangsheng,Chen, Rui,Yuan, Lilai,et al. Global microRNA and isomiR expression associated with liver metabolism is induced by organophosphorus flame retardant exposure in male Chinese rare minnow (Gobiocypris rarus)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,649:829-838.
APA	Hong, Xiangsheng,Chen, Rui,Yuan, Lilai,&Zha, Jinmiao.(2019).Global microRNA and isomiR expression associated with liver metabolism is induced by organophosphorus flame retardant exposure in male Chinese rare minnow (Gobiocypris rarus).SCIENCE OF THE TOTAL ENVIRONMENT,649,829-838.
MLA	Hong, Xiangsheng,et al."Global microRNA and isomiR expression associated with liver metabolism is induced by organophosphorus flame retardant exposure in male Chinese rare minnow (Gobiocypris rarus)".SCIENCE OF THE TOTAL ENVIRONMENT 649(2019):829-838.