Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China)

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China)
	Wang, Weidong ; Liu, Weiyue ; Wu, Di ; Wang, Xiaoxia ; Zhu, Guibing
刊名	JOURNAL OF SOILS AND SEDIMENTS
	2019-02-01
卷号	19 期号:2 页码:1005-1016
关键词	Abundance Eutrophic freshwater lake Littoral zone Microbial N-cycle Sediment
ISSN号	1439-0108
英文摘要	PurposeNitrogen (N) is one of the major elements causing eutrophication in freshwater lakes, and the N cycle is mainly driven by microorganisms. Lake littoral zones are found to be hotspots for N removal from both the basin and receiving waters. However, the environmental factors that drive the distribution of microorganisms are diverse and unclear. Here, we examined the differentiation of nitrogen and microbial community between the littoral and limnetic sediments to explore their interactions.Materials and methodsSediment samples were collected in the littoral and limnetic zones of Chaohu Lake in winter (ca. 7 degrees C) and autumn (ca. 22 degrees C). Abundances of the bacterial and archaeal genes amoA (ammoxidation), nirS and nirK (denitrification), hzsB (anaerobic ammonium oxidation; anammox), and nrfA (dissimilatory nitrate reduction to ammonium; DNRA) were measured via quantitative real-time polymerase chain reaction (qPCR). Clone libraries were constructed for further phylogenetic analysis to study the community composition.Results and discussionWe observed significant higher concentration values in terms of sedimentary NH4+-N and NO3--N in the limnetic zone than littoral zone (p<0.05; n=12). In general, abundance values of the above six genes in the littoral zone were all higher than those in the limnetic zone, while higher in winter (7 degrees C) than in autumn (22 degrees C) (p<0.05; n=6). The spatial heterogeneity had the most significant effect on the distribution of ammonia-oxidizing archaea (AOA) and anammox bacteria abundance. Both temporal (temperature) and spatial heterogeneity affected the abundance of ammonia-oxidizing bacteria (AOB). The variation in the abundance of denitrifying bacteria and DNRA bacteria mainly reflected the temporal (temperature) heterogeneity.ConclusionsThe six N-cycle-related microorganisms were affected by different environmental factors and presented different distribution patterns. The lower nitrogen content and the higher microbial abundance and diversity showed that the littoral zone was the hotspot of N-cycling-related microorganisms in a large, eutrophic, and turbid lake. It is suggested that increasing the area and restoring the ecological function of the littoral zone was effective and significant in eutrophic lake management.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43439]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weidong,Liu, Weiyue,Wu, Di,et al. Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China)[J]. JOURNAL OF SOILS AND SEDIMENTS,2019,19(2):1005-1016.
APA	Wang, Weidong,Liu, Weiyue,Wu, Di,Wang, Xiaoxia,&Zhu, Guibing.(2019).Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China).JOURNAL OF SOILS AND SEDIMENTS,19(2),1005-1016.
MLA	Wang, Weidong,et al."Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China)".JOURNAL OF SOILS AND SEDIMENTS 19.2(2019):1005-1016.