High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 水污染控制实验室

	High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system
	Chen, Yihan ; Su, Jian-Qiang ; Zhang, Junya ; Li, Peng ; Chen, Hongjie ; Zhang, Bo ; Gin, Karina Yew-Hoong ; He, Yiliang
刊名	WATER RESEARCH
	2019-02-01
卷号	149 页码:179-189
关键词	Antibiotic resistance genes High-throughput qPCR River-reservoir system Structural equation model Environmental gradient
ISSN号	0043-1354
英文摘要	The rapid construction of reservoir in river basin generates a river-reservoir system containing an environmental gradient from river system to reservoir system in modern aquatic environment worldwide. Profiles of antibiotic resistance genes (ARGs) in river-reservoir system is essential to better understand their dynamic mechanisms in aquatic eco-environment. In this study, we investigated the diversity, abundance, distribution of ARGs and mobile genetic elements (MGEs) in a representative river-reservoir system using high-throughput quantitative PCR, as well as ranked the factors (e.g. antibiotics, bacterial biomass, bacteria communities, and MGEs) influencing the patterns of ARGs based on structural equation models (SEMs). Seasonal variations in absolute abundance of ARGs and MGEs exhibited similar trends with local rainfall, suggesting that seasonal runoff induced by the rainfall potentially promote the absolute abundance of ARGs and MGEs. In contrast, environmental gradient played more important roles in the detected number, relative abundance, distribution pattern of ARGs and MGEs in the river-reservoir system. Moreover, environmental gradient also made the co-occurrence patterns associated with ARGs subtypes, MGEs and bacteria genera in river system different from those in reservoir system. The SEMs revealed that MGEs contributed the most to shape the ARG profiles. Overall, our findings provide novel insights into the mechanisms of environmental gradient on ARGs dynamics in river-reservoir system, probably via influencing the MGEs, antibiotics, pathogenic bacteria community and nonpathogenic bacteria community. (C) 2018 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43256]
专题	生态环境研究中心_水污染控制实验室
作者单位	1.Anhui Jianzhu Univ, Sch Environm & Energy Engn, Hefei 230601, Anhui, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, 800 Dongchuan Rd, Shanghai 200240, Peoples R China 3.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100085, Peoples R China 5.Natl Univ Singapore, NUS Environm Res Inst, 5A Engn Dr 1,02-01, Singapore 117411, Singapore
推荐引用方式 GB/T 7714	Chen, Yihan,Su, Jian-Qiang,Zhang, Junya,et al. High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system[J]. WATER RESEARCH,2019,149:179-189.
APA	Chen, Yihan.,Su, Jian-Qiang.,Zhang, Junya.,Li, Peng.,Chen, Hongjie.,...&He, Yiliang.(2019).High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system.WATER RESEARCH,149,179-189.
MLA	Chen, Yihan,et al."High-throughput profiling of antibiotic resistance gene dynamic in a drinking water river-reservoir system".WATER RESEARCH 149(2019):179-189.