Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota
	Ding, Jing ; Zhu, Dong ; Hong, Bin ; Wang, Hong Tao ; Li, Gang ; Ma, Yi Bing ; Tang, Yu Ting ; Chen, Qing Lin
刊名	ENVIRONMENT INTERNATIONAL
	2019-03
卷号	124 页码:145-152
关键词	Antibiotic resistome Gut microbiota Soil fauna Sewage sludge Chicken manure
ISSN号	0160-4120
英文摘要	Antibiotic resistance genes (ARGs), prevalent across multiple environmental media, threaten human health worldwide and are considered emerging environmental contaminants. Earthworm gut, a niche for bacteria to survive, represents a potential reservoir for ARGs in soil. However, the compositions of ARGs in the earthworm gut microbiota remain elusive, especially under field conditions. In this study, we applied high-throughput quantitative PCR to profile the ARGs in the gut microbiota of earthworms after chronic exposure to fertilizers. To elucidate the factors that impact the ARGs composition, the bacterial community of gut microbiota, mobile genetic elements (MGEs), soil (nutrients, heavy metals, and antibiotics) and the properties of gut content (pH and nutrients) were analyzed. A total of 98 subtypes among 9 major types of ARGs, and 3 different MGEs were detected in the gut microbiota of earthworms. Organic fertilizer (sewage sludge and chicken manure) application significantly increased the diversity and abundance of ARGs. Of the 1123 identified operational taxonomic units (OTUs) at 97% similarity cutoff, most of them were assigned to Firmicutes (55.5%) and Proteobacteria (33.6%) in earthworm gut microbiota. Long-term organic fertilization slightly changed the microbiota composition, but did not impact the diversity. Partial redundancy analysis (pRDA) revealed that bacterial community, combined with environmental factors (soil and gut content properties) and MGEs, explained 72% of the variations of ARGs in the earthworm gut. Furthermore, the co-occurrence pattern between ARGs and MGEs indicated that horizontal gene transfer via MGEs may occur in the earthworm gut. These findings improve the current understanding of the dynamics of soil fauna-associated ARGs and the gut microbiota of earthworms may be an underappreciated hotspot for ARGs in the environment.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42752]
专题	生态环境研究中心_城市与区域生态国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Plant Nutr & Nutrient Cycling, Minist Agr, Beijing 100081, Peoples R China 5.Univ Nottingham, Sch Geog Sci, Ningbo 315100, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jing,Zhu, Dong,Hong, Bin,et al. Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota[J]. ENVIRONMENT INTERNATIONAL,2019,124:145-152.
APA	Ding, Jing.,Zhu, Dong.,Hong, Bin.,Wang, Hong Tao.,Li, Gang.,...&Chen, Qing Lin.(2019).Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota.ENVIRONMENT INTERNATIONAL,124,145-152.
MLA	Ding, Jing,et al."Long-term application of organic fertilization causes the accumulation of antibiotic resistome in earthworm gut microbiota".ENVIRONMENT INTERNATIONAL 124(2019):145-152.