Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils
	Zhao, Yi ; Cocerva, Tatiana ; Cox, Siobhan ; Tardif, Stacie ; Su, Jian-Qiang ; Zhu, Yong-Guan ; Brandt, Kristian Koefoed
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-03-15
卷号	656 页码:512-520
关键词	Antibiotic resistance genes Mobile genetic elements Co-selection Metal toxicity qPCR chip Urban soils
ISSN号	0048-9697
英文摘要	Antibiotic resistance genes (ARGs) constitute emerging environmental pollutants and pose risks to public health. Toxic metals are known to select for metal-resistant bacteria in metal-contaminated soils, but there is growing concern that metal contaminants can also act as co-selective agents thereby causing environmental proliferation of antibiotic resistance. In this study, we quantified ARGs and selected mobile genetic elements (MGEs) known to constitute potential ARG hosts in 50 archived urban and suburban soils from the Belfast metropolitan area using a high-throughput qPCR ARG chip. ARG prevalence was linked to concentrations of individual metals and a soil metal toxicity index calculated based on the relative toxicity of different metals to soil microbial processes. A total of 164 ARGs were detected across the 50 soils analyzed with an average absolute abundance of 3.4 x 10(7) ARG gene copies per gram of soil. A significant correlation between abundance of ARGs and MGEs was observed, suggesting the importance of horizontal gene transfer for ARG dissemination. Network analysis revealed significant co-occurrence patterns between specificmetals (As, Cd, Co, Cr, Cu. Hg, Ni and Zn) and associated ARGs. Path analysis further indicated that the soil metal toxicity index significantly affected the number of detected ARGs (lambda = 0.32, P < 0.001) and the abundance of metal co-occurring ARGs (lambda = 0.612, P < 0.001) via effects on MGEs. Collectively, our results indicate a role of soil metals in co-selection of ARGs and MGEs in urban and semi-urban soils and suggest a risk for environmental ARG dissemination via horizontal gene transfer. (C) 2018 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42727]
专题	生态环境研究中心_城市与区域生态国家重点实验室
作者单位	1.Univ Copenhagen, Dept Plant & Environm Sci, Fac Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark 2.Queens Univ Belfast, Sch Nat & Built Environm, David Keir Bldg,Stranmillis Rd, Belfast BT9 6AX, Antrim, North Ireland 3.Chinese Acad Sci, Key Lab Urban Environm & Hlth, Inst Urban Environm, 1799 Jimei Rd, Xiamen 361021, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 5.Sinodanish Ctr Educ & Res, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yi,Cocerva, Tatiana,Cox, Siobhan,et al. Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,656:512-520.
APA	Zhao, Yi.,Cocerva, Tatiana.,Cox, Siobhan.,Tardif, Stacie.,Su, Jian-Qiang.,...&Brandt, Kristian Koefoed.(2019).Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils.SCIENCE OF THE TOTAL ENVIRONMENT,656,512-520.
MLA	Zhao, Yi,et al."Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils".SCIENCE OF THE TOTAL ENVIRONMENT 656(2019):512-520.