High contribution of ammonia-oxidizing archaea (AOA) to ammonia oxidation related to a potential active AOA species in various arable land soils

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境水质学国家重点实验室

	High contribution of ammonia-oxidizing archaea (AOA) to ammonia oxidation related to a potential active AOA species in various arable land soils
	Wang, Mengzi ; Wang, Shanyun ; Long, Xien ; Zhuang, Linjie ; Zhao, Xue ; Jia, Zhongjun ; Zhu, Guibing
刊名	JOURNAL OF SOILS AND SEDIMENTS
	2019-03
卷号	19 期号:3 页码:1077-1087
关键词	Activity Arable land soil Archaeal ammonia oxidation Bacterial ammonia oxidation Nitrosocosmicus
ISSN号	1439-0108
英文摘要	PurposeAmmonia oxidation is the limiting step in soil nitrification and critical in the global nitrogen cycle. The discovery of ammonia-oxidizing archaea (AOA) has improved our knowledge of microbial mechanisms for ammonia oxidation in complex soil environments. However, the relative contributions of AOA and ammonia-oxidizing bacteria (AOB) to ammonia oxidation remain unclear.Materials and methodsIn this study, through large geographical scale sampling in China, totally nine samples representing various types of arable land soils were selected for analyzing the ammonia oxidation activity. The AOA and AOB activities were separately determined by using the dicyandiamide and 1-octyne inhibition method. High-throughput pyrosequencing and DNA stable-isotope probing (DNA-SIP) analysis were applied to investigate the distribution and activity of Candidatus Nitrosocosmicus franklandus in the arable land soils.Results and discussionIn this study, AOA abundance (3.2x10(7)-3.4x10(9) copies g(-1)) and activity (0.01-1.33mgNkg(-1) dry soilday(-1)) were evaluated for nine selected arable land soils and accounted for 4-100% of ammonia oxidation. By separately determining AOA and AOB rates, we observed that archaeal ammonia oxidation dominated the ammonia oxidation process in six soils, revealing a considerable contribution of AOA in ammonia oxidation in arable land soils. Based on high-throughput pyrosequencing analysis, the AOA species Ca. N. franklandus with relatively low abundance (0.6-13.5% in AOA) was ubiquitously distributed in all the tested samples. Moreover, according to the DNA-SIP analysis for Urumqi sample, the high activity and efficiency of Ca. N. franklandus in using CO2 suggests that this species plays an important role in archaeal ammonia oxidation in arable land soils.ConclusionsThrough determining the AOA activity and analyzing the potential predominant functional AOA species, this study greatly improves our understanding of ammonia oxidation in arable land soils.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43089]
专题	生态环境研究中心_环境水质学国家重点实验室
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Xiamen 361021, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Jiangsu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Mengzi,Wang, Shanyun,Long, Xien,et al. High contribution of ammonia-oxidizing archaea (AOA) to ammonia oxidation related to a potential active AOA species in various arable land soils[J]. JOURNAL OF SOILS AND SEDIMENTS,2019,19(3):1077-1087.
APA	Wang, Mengzi.,Wang, Shanyun.,Long, Xien.,Zhuang, Linjie.,Zhao, Xue.,...&Zhu, Guibing.(2019).High contribution of ammonia-oxidizing archaea (AOA) to ammonia oxidation related to a potential active AOA species in various arable land soils.JOURNAL OF SOILS AND SEDIMENTS,19(3),1077-1087.
MLA	Wang, Mengzi,et al."High contribution of ammonia-oxidizing archaea (AOA) to ammonia oxidation related to a potential active AOA species in various arable land soils".JOURNAL OF SOILS AND SEDIMENTS 19.3(2019):1077-1087.