A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates

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	A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates
	Liu, Yongqiang ; Nan, Bei ; Duan, Lili ; Cheng, Ting ; Bourland, William A.; Liu, Mingjian ; Zhao, Yan
刊名	JOURNAL OF EUKARYOTIC MICROBIOLOGY
	2019-09-01
卷号	66 期号:5 页码:836-848
关键词	Freezing liquid nitrogen methodology protozoa recovery efficiency
ISSN号	1066-5234
英文摘要	Pseudocohnilembus persalinus is a free-living marine scuticociliate that, as a new model organism, has been used in a wide variety of studies. However, long-term laboratory maintenance for this species is mainly achieved by subculture that requires rigorous culture environments and, too often, cultures of the organism die out for a variety of reasons. Successful transport of viable cultures also poses problems for researchers. This study describes a simple and rapid protocol for long-term cryopreservation of P. persalinus. The effects of physiological states of individuals before freezing, the type and concentration of cryoprotectant, and optimal temperatures for freezing and thawing were assessed. A cryopreservation protocol, using a mixture of 30% glycerol and 70% concentrated P. persalinus cell culture, incorporating rate-controlled freezing at -80 degrees C before liquid nitrogen storage, maintained a high recovery efficiency after 8 wk of storage. These results suggest that broader application of this protocol to build a cryopreserved marine protozoa culture bank for biological studies may be possible.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42619]
专题	生态环境研究中心_环境水质学国家重点实验室
作者单位	1.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Shandong, Peoples R China 2.Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yongqiang,Nan, Bei,Duan, Lili,et al. A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2019,66(5):836-848.
APA	Liu, Yongqiang.,Nan, Bei.,Duan, Lili.,Cheng, Ting.,Bourland, William A..,...&Zhao, Yan.(2019).A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates.JOURNAL OF EUKARYOTIC MICROBIOLOGY,66(5),836-848.
MLA	Liu, Yongqiang,et al."A Simple and Rapid Cryopreservation Technique for Ciliates: A Long-Term Storage Procedure Used for Marine Scuticociliates".JOURNAL OF EUKARYOTIC MICROBIOLOGY 66.5(2019):836-848.