Type 3 innate lymphoid cells are altered in colons of C57BL/6 mice with dioxin exposure

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Type 3 innate lymphoid cells are altered in colons of C57BL/6 mice with dioxin exposure
	Li, Yunping ; Xie, Heidi Qunhui ; Zhang, Wanglong ; Wei, Yunbo ; Sha, Rui ; Xu, Li ; Zhang, Jianqing ; Jiang, Yousheng ; Guo, Tai L.; Zhao, Bin
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019-04-20
卷号	662 页码:639-645
关键词	TCDD ILC3 ROR gamma t IL-17a IFN-gamma
ISSN号	0048-9697
英文摘要	Type 3 innate lymphoid cells (ILC3s) are distributed in the gut and regulate inflammation by secreting cytokines, including interferon (IFN)-gamma and interleukin (IL)-17. The maintenance and function of ILC3s involve the activity of aryl hydrocarbon receptor (AhR), a potent ligand of which is 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), one of the most toxic dioxin congeners. Thus, TCDD exposure might affect ILC3s. To obtain in vivo evidence supporting this notion, we exposed female C57BL/6 mice orally to TCDD (low/high doses: 0.1/10 mu g/kg body weight) during pregnancy and lactation periods, and after the exposure, evaluated the mothers and offspring for alterations in ILC3 differentiation and function in the colon. ILC3 frequency among colonic lamina propria lymphocytes was preferentially diminished in the offspring, and, in parallel, the median fluorescence intensity (MFI) of retinoic acid receptor-related orphan receptor (ROR)gamma t, which is associated with ILC3 differentiation, was also decreased in ILC3s. Conversely, the percentages of two subsets of the cells, one positive for natural cytotoxicity receptor NKp46 and the other for IL-17a, were increased in TCDD-exposed mothers and offspring. Moreover, the percentage of IFN-gamma(+) ILC3s was increased specifically in the mothers, but this was in conjunction with a significant decrease in the MFI of IFN-gamma, which suggests that the IFN-gamma(+) ILC3 subset was functionally altered. In conclusion, maternal exposure to TCDD suppresses ILC3 differentiation in the offspring and influences ILC3 function in distinct manners in the mother and offspring. Our study provides new insights into the intergenerational interference of dioxins in colonic ILC3s. (C) 2019 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/43048]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Shenzhen Ctr Dis Control & Prevent, Shenzhen 518055, Peoples R China 2.Univ Georgia, Dept Vet Biosci & Diagnost Imaging, Coll Vet Med, Athens, GA 30602 USA 3.Shandong Acad Sci, Qilu Univ Technol, Shandong Anal & Test Ctr, Lab Immunol Environm & Hlth, Jinan 250014, Shandong, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yunping,Xie, Heidi Qunhui,Zhang, Wanglong,et al. Type 3 innate lymphoid cells are altered in colons of C57BL/6 mice with dioxin exposure[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,662:639-645.
APA	Li, Yunping.,Xie, Heidi Qunhui.,Zhang, Wanglong.,Wei, Yunbo.,Sha, Rui.,...&Zhao, Bin.(2019).Type 3 innate lymphoid cells are altered in colons of C57BL/6 mice with dioxin exposure.SCIENCE OF THE TOTAL ENVIRONMENT,662,639-645.
MLA	Li, Yunping,et al."Type 3 innate lymphoid cells are altered in colons of C57BL/6 mice with dioxin exposure".SCIENCE OF THE TOTAL ENVIRONMENT 662(2019):639-645.