A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment
	Chen, Baowei ; Fang, Linchuan ; Yan, Xueting ; Zhang, Aiqian ; Chen, Ping ; Luan, Tiangang ; Hu, Ligang ; Jiang, Guibin
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2019-02-05
卷号	363 页码:34-40
关键词	Lead Biosorption Metal-binding protein Microbes
ISSN号	0304-3894
英文摘要	Metal contaminants present persistent and deleterious threats to environmental ecosystems and human health. Microorganisms can rapidly develop protective mechanisms against metal toxicity, such as metallothionein production. The identification of biological factors related to these protective mechanisms is essential for effective metal remediation. This study presents a robust pathway to rapidly locate and characterize a Pb-binding flagellin in Serratia Se1998, which can bind Pb at a 16:1 Pb: protein ratio. A column gel electrophoresis system hyphenated with inductively coupled plasma mass spectrometry (ICP MS) was constructed to efficiently separate and identify Pb-binding proteins from the whole bacterial proteome. PCR and transgenic assays were used to elucidate the exact sequences and biological function of Pb-binding proteins and heterogeneous expression of Pb-binding flagellin in E. colt could significantly enhance Pb removal from aqueous solution by approximately 45%. This method provides a benchmark procedure to rapidly identify biological factors responsible for metal bio-sorption. Identification of this unique Pb-binding flagellin highlights that microorganisms can survive high metal stresses due to various complex biological pathways for metal detoxification and remediation.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42888]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Sun Yat Sen Univ, Sch Marine Sci, Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou 510275, Guangdong, Peoples R China 2.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China 5.Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Baowei,Fang, Linchuan,Yan, Xueting,et al. A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,363:34-40.
APA	Chen, Baowei.,Fang, Linchuan.,Yan, Xueting.,Zhang, Aiqian.,Chen, Ping.,...&Jiang, Guibin.(2019).A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment.JOURNAL OF HAZARDOUS MATERIALS,363,34-40.
MLA	Chen, Baowei,et al."A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment".JOURNAL OF HAZARDOUS MATERIALS 363(2019):34-40.