Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair
	Zhang, Dapeng 2; Wang, Hailin 2
刊名	ANALYTICAL CHEMISTRY
	2019-11-19
卷号	91 期号:22 页码:14538-14544
ISSN号	0003-2700
英文摘要	Silver is a common heavy metal, and the detection of silver ion (Ag+) is of great importance because of its wide application and hazardous effect on the environment and human health. However, it is a great challenge to produce a large fluorescence anisotropy (FA) change for small molecules (e.g, Ag+ Herein, we describe a novel fluorescence anisotropy reduction approach for the sensitive and specific detection of Ag+. The feasibility of this method is demonstrated through screening a number of guanine-rich oligonucleotide probes. By selectively labeling the oligonucleotides with a single fluorophore tetramethylrhodamine (TMR), the reduction in FA response is associated with the conformation change from the unfolded to a hairpin-like folded structure by inducing formation of the intermolecular G-Ag+-G base pair, which diminishes the interaction between guanine and TMR by photoinduced electron transfer (PET). The change in FA allows the selective detection of Ag+ at a concentration as low as 0.5 nM with a dynamic range from 2.0 to 100 nM. The interference from the other 14 metal ions with a 100-fold even to a 1000-fold excess amount is negligible. This simple and cost-effective probe was further explored to determine cysteine (Cys) based on competing with a guanine-rich oligonucelotide for Ag+-binding.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42543]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dapeng,Wang, Hailin. Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair[J]. ANALYTICAL CHEMISTRY,2019,91(22):14538-14544.
APA	Zhang, Dapeng,&Wang, Hailin.(2019).Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair.ANALYTICAL CHEMISTRY,91(22),14538-14544.
MLA	Zhang, Dapeng,et al."Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair".ANALYTICAL CHEMISTRY 91.22(2019):14538-14544.