Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities
	Yin, Nuoya 3; Liang, Xiaoxing 3; Liang, Shengxian 3; Lan, Shaojun 3; Yang, Renjun 3; Hu, Bowen 3; Cheng, Zhanwen 3; Liu, Shuyu 2,3; Dong, Hengzhi 3; Liu, Sijin 3
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2019-07-30
卷号	176 页码:330-338
关键词	Mouse embryonic stem cells (mESCs) Bisphenols A, F and S Developmental toxicity Stem cell toxicology
ISSN号	0147-6513
英文摘要	Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differ. entiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/42536]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
作者单位	1.Univ Cambridge, Wellcome Trust CRUK Gurdon Inst, Dept Pathol, Cambridge CB2 1QN, England 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Yin, Nuoya,Liang, Xiaoxing,Liang, Shengxian,et al. Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,176:330-338.
APA	Yin, Nuoya.,Liang, Xiaoxing.,Liang, Shengxian.,Lan, Shaojun.,Yang, Renjun.,...&Faiola, Francesco.(2019).Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,176,330-338.
MLA	Yin, Nuoya,et al."Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 176(2019):330-338.