Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles

doi:10.1039/d0ay00800a

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	Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles
	Zeng, Yan 1,2,3; Qi, Peng 1,2,3; Zhang, Dun 1,2,3
刊名	ANALYTICAL METHODS
	2020-07-14
卷号	12 期号:26 页码:3404-3410
ISSN号	1759-9660
DOI	10.1039/d0ay00800a
通讯作者	Qi, Peng() ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要	The level ofd-amino acids (DAAs) is significantly related to bacterial contamination in health and disease, food science and nutrition, and industrial applications. Most sensing methods for the detection of DAAs need expensive equipment, and skilled operation experience, making the test kit for DAA analysis much more complex and challenging. Toward this end, we exploited a label-free DAA test kit based on 1,4-benzenediboronic acid (BDBA)-induced gold nanoparticles (AuNPs) aggregations. DAAs were first catalyzed by their specific catalase (DAAO,d-amino acid oxidase) and oxidized to produce H2O2. Then, the produced H(2)O(2)inhibited the citrate-capped AuNPs aggregation under BDBA, while the unreacted BDBA could lead to AuNPs aggregation. As a result, the UV/vis absorption spectra and optical photographs of the AuNPs solution are changed in the presence of different DAA target amounts. This method not only can be used for qualitative and semi-quantitative analysis with a naked-eye readout, but also it can quantify DAAs in aqueous solutions with high sensitivity and specificity. Furthermore, the DAAs test kit provided an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples. We believe this DAA test kit provides the potential to be further developed for DAA detection for satisfying both lab and practical needs in different fields.
资助项目	National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[51771180] ; National Natural Science Foundation of China[41906037] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000546454400012
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167816]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Qi, Peng; Zhang, Dun
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Yan,Qi, Peng,Zhang, Dun. Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles[J]. ANALYTICAL METHODS,2020,12(26):3404-3410.
APA	Zeng, Yan,Qi, Peng,&Zhang, Dun.(2020).Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.ANALYTICAL METHODS,12(26),3404-3410.
MLA	Zeng, Yan,et al."Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles".ANALYTICAL METHODS 12.26(2020):3404-3410.