New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments

doi:10.3389/fmicb.2020.01102

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments
	Wei, Miao 3,4; Xu, Kuidong 1,2,3,4
刊名	FRONTIERS IN MICROBIOLOGY
	2020-05-29
卷号	11 页码:15
关键词	viral abundance prokaryotic abundance virus-to-prokaryote ratio virus-host relationship marine sediment
ISSN号	1664-302X
DOI	10.3389/fmicb.2020.01102
通讯作者	Xu, Kuidong(kxu@qdio.ac.cn)
英文摘要	The virus-to-prokaryote ratio (VPR), which reflects the numerical dominance of viruses over their hosts, has been proposed as a proxy for assessing the relationship between viruses and prokaryotes. Previous studies showed that VPR values fluctuate over six orders of magnitude within and across various benthic ecosystems, with an average value of approximately 10. We hypothesize that this high VPR value is largely due to the inaccurate enumeration of viruses and prokaryotes (e.g., centrifugation treatments may lead to a three-fourfold overestimation of VPR). In this study, we evaluated the impact of processing methods on the determination of VPR values. Using an optimized procedure, we investigated the marine benthic VPR at 31 sites, from intertidal zones through continental shelves to abyssal plains, and assessed its monthly variation in two contrasting intertidal habitats (muddy-sand and sandy). By compiling 135 VPR data points of surface sediments from 37 publications, we reveal the effect of centrifugation on published VPR values and describe the spatial distribution of VPR values on a larger scale based on reliable data. The results showed that the commonly used centrifugation method may result in an overestimation of VPR values that are approximately one order of magnitude higher than those obtained using the dilution method, while other processing steps had a limited impact on the VPR. Our analysis indicates that the benthic VPR value is low and less varied across temporal and spatial scales, fluctuating mostly within 10, and the average VPR is approximately 2 in both marine and freshwater habitats. An insignificant seasonal pattern in the VPR was observed in the intertidal zone, with lower VPR values occurring at high temperatures. The VPR spatial distribution was primarily associated with sediment phaeophytin a, suggesting that the trophic conditions of the upper water column and the sedimentation of organic matter to the bottom are the key factors affecting VPR values. The mean VPR in benthic habitats is approximately one order of magnitude lower and much less varied than that observed in pelagic habitats, indicating that the virus-host relationship and the ecological function of viruses in the two ecosystems may be very different.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA19060401] ; Key Program of National Natural Science Foundation of China[41930533]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000542118700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167768]
专题	海洋研究所_海洋生物分类与系统演化实验室
通讯作者	Xu, Kuidong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wei, Miao,Xu, Kuidong. New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments[J]. FRONTIERS IN MICROBIOLOGY,2020,11:15.
APA	Wei, Miao,&Xu, Kuidong.(2020).New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments.FRONTIERS IN MICROBIOLOGY,11,15.
MLA	Wei, Miao,et al."New Insights Into the Virus-to-Prokaryote Ratio (VPR) in Marine Sediments".FRONTIERS IN MICROBIOLOGY 11(2020):15.