Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea

doi:10.1016/j.ecss.2020.106887

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea
	Chen, Tiantian 1,3,4; Liu, Yun 1,3,4; Xu, Sha 1,2,3; Song, Shuqun 1,3,4; Li, Caiwen 1,2,3,4
刊名	ESTUARINE COASTAL AND SHELF SCIENCE
	2020-09-30
卷号	243 页码:11
关键词	Harmful algal blooms Dinoflagellate Parasitism High-throughput sequencing qRT-PCR
ISSN号	0272-7714
DOI	10.1016/j.ecss.2020.106887
通讯作者	Li, Caiwen(cwli@qdio.ac.cn)
英文摘要	The parasitic dinoflagellates in the genus Amoebophrya infect and kill a number of free-living dinoflagellates. The parasitoid has the potential to regulate harmful dinoflagellate blooms due to its high offspring production rates and a certain degree of host specificity. The planktonic dinoflagellate Prorocentrum donghaiense Lu is a commonly observed harmful algal species in coastal waters of China, and limited studies have been conducted to explore the ecological roles of parasitism in association with bloom dynamics. Thus, the in situ occurrence, prevalence, and dynamics of Amoebophrya during the recurrent blooms of P. donghaiense were systemically studied via morphological observations and high-throughput sequencing. The parasitic infections were identified microscopically in six dinoflagellate taxa with prevalence up to 5.13%, while Amoebophrya infection in the bloomforming species P. donghaiense was rare (<0.01%), suggesting that P. donghaiense was free of the top-down control of Amoebophrya in the area. In addition, high-throughput sequencing of environmental DNA revealed that Amoebophrya co-dominated both the initiation and termination stages of the P. donghaiense bloom (up to 21.20% of OTU), but was less abundant during persistence of the bloom (3.70% of OTU). Further, the dynamics of Amoebophrya in the water column possessed similar patterns as revealed by qRT-PCR, in which high copy numbers of Amoebophrya (up to 3.60 x 10(9) copies L-1) were detected in the initiation and termination stages of the P. donghaiense bloom and low copy numbers (1.16 x 10(9) copies L-1) during persistence of the bloom. These findings manifested the paradoxical dynamics and complicated ecological strategies of Amoebophrya organisms during dinoflagellate blooms that need further study.
资助项目	National Natural Science Foundation of China (NSFC)[41906122] ; National Natural Science Foundation of China (NSFC)[48176120] ; National Natural Science Foundation of China (NSFC)[41606128] ; China Postdoctoral Science Foundation[2018M642709] ; National Key R&D Program of China[2017YFC1404300] ; Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504-2]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000570081600003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168786]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Li, Caiwen
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingda 266200, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tiantian,Liu, Yun,Xu, Sha,et al. Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea[J]. ESTUARINE COASTAL AND SHELF SCIENCE,2020,243:11.
APA	Chen, Tiantian,Liu, Yun,Xu, Sha,Song, Shuqun,&Li, Caiwen.(2020).Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea.ESTUARINE COASTAL AND SHELF SCIENCE,243,11.
MLA	Chen, Tiantian,et al."Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea".ESTUARINE COASTAL AND SHELF SCIENCE 243(2020):11.